NZY Tissue gDNA Isolation kits are designed for the simple and rapid small-scale preparation of highly pure genomic DNA from a variety of sample sources including animal cells and tissues, Gram-positive and Gram-negative bacteria, mouse tails, yeast, forensic samples and clinical samples.
The method is spin column silica-based and requires no phenol or chloroform extraction. This kit uses optimized lysis buffers containing Proteinase K and SDS to release DNA from cells. After preparing the lysate, DNA is selectively absorbed into the NZYSpin Tissue Column and others impurities such as proteins and salts are removed during the washing steps. The eluted genomic DNA has a A260/280 ratio between 1.7 and 1.9 what makes it ready to use in applications like sequencing, PCR, multiplex-PCR, genotyping and a wide range of other enzymatic manipulations.
Features:
– High genomic DNA quality
– High yields from multiple sample sources
– Optimized protocols for a range of starting materials
– Fast and easy small-scale protocol
– Silica-membrane technology
– RNAse A included
Applications:
The purified genomic DNA is suitable for use in the most demanding molecular biology applications, including:
– PCR
– Real-time quantitative PCR (qPCR)
– Genotyping
– DNA sequencing
– Multiplex-PCR
– Enzimatic manipulations
Specifications:
| Sample material (general): | tissue, bacterial cells, blood |
| Format: | Spin-column |
| Sample amount: | < 25 mg tissue/107 cells |
| Typical yield: | up to 35 µg |
| A260/A280: | 1.7-1.9 |
| Elution volume: | 60-100 µL |
| Binding capacity: | 60 µg |
| Preparation time | 20 min/preparation (excluding lysis step) |
Components:
– Buffer NT1
– Buffer NL
– Buffer NW1
– Buffer NW2 (concentrated)
– Buffer NE
– Proteinase K (lyophilized)
– Proteinase buffer
– RNase A (lyophilized)
– NZYSpin Tissue columns
– Collection tubes
Citations
The Chlamydia trachomatis type III secretion substrates CT142, CT143, and CT144 are secreted into the lumen of the inclusion
da Cunha M, Pais SV, Bugalhão JN, Mota LJ
PLoS One, 2017
Fernandes AP, Nunes TC, Paquete CM, Salgueiro CA.
Biochem J, 2017
Covelo-Soto L, Saura M, Morán P
Comp Biochem Physiol B Biochem Mol Biol, 2015
