NZY Viral RNA Isolation kit

Description: NZY Viral RNA Isolation kit is designed for the rapid and simple purification of viral RNA of the highest integrity from a variety of sources, in particular serum, plasma, saliva, nasal samples, blood, tissue, organs and environmental samples. This method uses a denaturing lysis buffer containing guanidine thiocyanate, which also inactivates cellular RNases, to ensure the recovery of intact RNA molecules.

Ethanol is subsequently added to provide selective binding of nucleic acids into the silica membrane column and impurities are efficiently washed away. High-quality viral RNA is then eluted in RNase-free water. Viral RNA is ready to use for detection using different amplification strategies such as Reverse Transcription (RT)-PCR or RT-qPCR.

NZY Viral RNA Isolation kit is optimized to isolate RNA with high purity/integrity displaying an A260/280 ratio between 1.9 and 2.1. Traces of DNA may be co-purified with the viral RNA. If a DNA-free sample is necessary for your downstream applications, please treat it with DNase to ensure the complete removal of DNA trace contaminations.

Features:
– High RNA quality
– Viral RNA from multiple sample sources (serum, plasma, saliva, nasal samples, blood, tissue, organs and environmental samples)
– Optimized protocols for a range of starting materials
– High-speed protocol
– Silica-membrane technology

Applications:
The purified RNA is suitable for use in the most demanding molecular biology applications, including:
– Reverse transcriptase PCR (RT-PCR)
– Real-time quantitative PCR (qPCR)
– cDNA synthesis

Specifications:

Components:
– Buffer NVL
– Buffer NV
– Buffer NVW (concentrated)
– RNase free water
– NZYSpin Virus Columns
– Collection tubes (2 mL)