NZYMaxiprep

NZYMaxiprep kit is designed for the rapid, large-scale preparation of highly pure plasmid DNA (typically 500 µg) from recombinant Escherichia coli strains. Plasmid DNA binds selectively to NZYTech columns charged with a silica-based anion-exchange resin. All contaminants, such as proteins, RNA, salts, nucleotides and oligos (<40-mer) are washed from the column. In the elution step, the positive charge of the resin is neutralized by a pH shift to slightly alkaline conditions and pure plasmid DNA is eluted in a high-salt elution buffer. To isolate DNA from low copy number plasmids, BACs or cosmids, or to obtain higher DNA concentrations, use 500 mL of E. coli cultures and double the volumes of Buffers M1, M2 and M3.

Features:
– Quick maxi-prep of pure plasmid DNA
– Column silica-based
– High yields of plasmid DNA (800-1000 µg of high-copy number plasmid DNA/ column)

Applications:
The purified plasmid DNA is suitable for use in the most demanding molecular biology applications, including:
– Transfection
– In vitro transcription
– Enzymatic reactions
– Automated fluorescent or manual sequencing
– PCR
– Cloning
– Hybridization


Specifications:

– Culture volume/starting material: 100-500 mL E. coli culture volume
– Plasmid type: high- and low-copy number, BACs or cosmids
– Format: gravity flow column
– Yield: up to 1000 µg
– A260/A280: 1.8–1.9
– Elution volume: variable
– Procedure: Manual – centrifugation
– Preparation time: approx. 70 min
– Storage conditions: All kit components can be stored at room temperature; Buffer M1 with RNase should be stored at 4 °C for frequent use
– Shipping conditions: Shipped at room temperature

Components (*):
– Buffer M1
– Buffer M2
– Buffer M3
– Buffer MEQ
– Buffer MW
– Buffer ME
– RNase A
– NZYTech Plasmid Maxi Columns

(*) For most convenience, NZYTech also sells in separate the buffers (NZYMaxiprep, no columns) and NZYTech Plasmid Maxi Columns