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NZYMiniprep 96 well plate

nr kat.: MB20201
Opakowanie: 2x 96 preps.
Cena brutto: 2 589,03 zł 2589.03
Cena netto: 2 104,90 zł
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Producent: NZYTech
nr kat.: MB20201

Opis

Description:

NZYMiniprep 96 well plate kit is designed for the manual or automated rapid, smallscale preparation of highly pure plasmid DNA from recombinant Escherichia coli strains. The method is based on the alkaline lysis of bacterial cells followed by adsorption of DNA onto silica in the presence of high salt. The plasmid DNA is selectively adsorbed in the silica gel-based NZYMiniprep 96 well plate and other impurities such as proteins, salts, nucleotides and oligos (<40-mer) are washed away. NZYMiniprep 96 well plate kit includes an additional washing buffer (AY) which is strongly recommended for the complete removal of high levels of endonucleases. NZYMiniprep 96 well plate kit allows simultaneous processing of up to 96 samples, typically in less than 45 min. Actual processing times may depend on the configuration of the liquid handling system used. The final concentration of the eluted DNA is 50–200 ng/μL (depending on the elution buffer volume and the bacterial culture). The kit is designed for use with vacuum manifolds or under centrifugation.

Features:
– Time-saving (allows isolating multiple highly pure plasmid DNA minipreps simultaneous)
– Suitable for high-throughput approaches
– Prevents the use of toxic substances (e.g. phenol, chloroform or CsCl)
– Contains an endonuclease-removal buffer (AY washing buffer)
– Protocol compatible with vacuum manifold and centrifugation

Applications:
The purified plasmid DNA is suitable for use in the most demanding molecular biology applications, including:
– Cloning
– Automated fluorescent or manual sequencing
– PCR
– In vitro transcription
– Enzymatic reactions
– Hybridization
– Transfection

Specifications:

Culture volume/starting material: 1-5 mL E. coli culture volume
Plasmid type: high- and low-copy number
Format: 96-well plate
Yield: 5-15 µg
A260/A280: 1.8–2.0
Elution volume:
75-150 μL (125 μL preferably)
Binding capacity: 20 µg/ well
Procedure: Manual or automated – under vacuum or centrifugation (see Figures & Tables above)
Preparation time: approx. 45 min (up to 96 samples)
Product size: 2 and 4×2 plates
Storage conditions: All kit components can be stored at room temperature; Buffer A1 with RNase should be stored at 4 °C for frequent use
Shipping conditions: Shipped at room temperature

Components:
– Buffer A1
– Buffer A2
– Buffer A3
– Buffer AY
– Buffer A4 (concentrate)
– Buffer AE (does not contain EDTA)
– RNase A
– NZY Plasmid filter plate
– NZY Plasmid binding plate
– Elution plate
– 96-deep-well block

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Opakowanie 2x 96 preps.
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