NZYProof DNA Polymerase
Opis
NZYProof DNA polymerase is a recombinant DNA polymerase that presents high fidelity and displays great performance in the majority of PCR applications. NZYProof DNA polymerase possesses 3´→5´ exonuclease proofreading capacity which enables the polymerase to amplify DNA with increased accuracy.
The enzyme is highly efficient in the amplification of longer (≤10 kb) PCR products and site-directed mutagenesis. In addition, it the recommended polymerase for routine cloning that requires precision. The error rate of NZYProof DNA polymerase is similar to that of Pfu and Kod DNA polymerases and significantly lower than the error rate of Taq DNA polymerases. NZYProof DNA polymerase generates blunt-ended PCR products that are suitable for cloning with NZYTech´s NZY-blunt PCR cloning kit (MB121).
Features:
- Amplifies DNA fragments up to 10 Kb
- Proofreading activity
- Enhanced sensitivity and yield with 5× Stabilizer Solution
Applications:
- High-fidelity PCR
- Site-directed mutagenesis
- Generation of products for blunt-end cloning
Enhanced sensitivity and yield of NZYProof DNA polymerase
NZYProof DNA polymerase has been optimized to allow amplification with high yield and sensitivity. Inclusion of 5× Stabilizer Solution greatly enhances the success of PCR.
A 1 kb DNA fragment was amplified using 2-fold Escherichia coli genomic DNA dilutions ranging from 100 to 1.56 ng (lanes 1-7) with NZYProof DNA polymerase plus 5× Stabilizer Solution (A) and NZYProof DNA polymerase without stabilizer (B). PCR was performed using 2.5 U of enzyme, 0.4 mM dNTPs and 0.45 µM of each primer. NC: No template control. Lane M: NZYDNA Ladder III (MB044).
Comparison of PCR products generated using NZYProof DNA polymerase with (+) and without (No) 5× Stabilizer Solution. Amplification of three E. coli genomic DNA fragments of different lengths (0.5, 1 and 2.5 kb) using 40 and 10 ng of template DNA. Lane M: NZYDNA Ladder III (MB0441).
Specifications:
- Product length: 0-10 kb
- Hot-start like capacity: no
- Extension time: 60 sec/kb at 72 °C
- 3´→5´ activity: yes
- Product overhang: blunt
- Available as Mixes: NZYProof 2x Green Master Mix (MB287) NZYProof 2x Colourless Master Mix (MB288)
- Storage conditions: Store at -20 ºC
- Shipping conditions: Shipped at 4 ºC to dry ice
Components:
- NZYProof DNA polymerase (2.5 U/μL)
- Reaction buffer (10x)
- Stabilizer Solution (5x)
Dane techniczne
Opakowanie | 500 U |