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NZYTaq II Polymerase with 5x Green Gel Load Reaction Buffer

Cena brutto: 288,36 zł
Cena netto: 267,00 zł
szt.
Opakowanie:
500 U
Kod produktu:

MB36401

Producent:

NZYTaq II DNA polymerase belongs to a new generation of Taq-derived DNA polymerases that was engineered to produce high DNA yields in shorter PCR running times (15-30 s/kb extension) under minimal optimization conditions. NZYTaq II DNA polymerase supports the robust and reliable amplification of a wide range of DNA templates up to 6 kb.

NZYTaq II is provided with 5× Gel Load Reaction Buffer allowing reactions to be loaded directly into gels without the extra adding of loading dye. This Gel Load Reaction Buffer is composed by a blue and yellow dye. The blue dye migrates at the same rate as a 3-5 kb DNA fragment in a 1% (w/v) agarose gel. The yellow dye migrates at a rate faster than primers (<50 bp) in a 1% (v/v) agarose gel. The 5× Gel Load Reaction Buffer is not suitable when direct fluorescent or absorbance readings are required without prior purification of the amplified DNA from PCR.

NZYTaq II DNA polymerase lacks 3´→5´ exonuclease activity. Resulting PCR products have an A-overhang and are suitable for cloning with NZYTech´s NZY-A PCR cloning kit (MB053) or NZY-A Speedy PCR cloning kit (MB137).

Features:
– Suitable for immediate loading onto agarose gels
– Robust amplification using a wide range of DNA templates up to 6kb
– Leaves an A-overhang

Applications:
– Powerful PCR
– Generation of products for TA cloning

Specifications:

Product length: 0-6 kb
Hot-start-like capacity: no
Extension time: 15-30 sec/kb at 72 ºC
3´→5´ activity: no
Product overhang: 3’-A
Storage conditions: Store at -20 ºC
Shipping conditions: Shipped at 4 ºC to dry ice

Components:
– NZYTaq II DNA polymerase (5 U/μL)
– Gel Load Reaction buffer (5x)
– MgCl2 Solution (50 mM)

EP0712

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