OncoSpan

Format: Genomic DNA

OncoSpan is the largest and most extensive oncology Reference Standard to date, featuring 386 variants across 152 key cancer genes.

In an exciting new first for Horizon, OncoSpan is provided with batch-specific NGS data, giving you knowledge and confidence of the background genotype of this cell-line-derived Reference Standard. OncoSpan’s larger gene list and better characterisation empowers users to perform more comprehensive validation of large oncology gene panels and exome sequencing assays than ever before.

OncoSpan is a well-characterised, cell line-derived Reference Standard containing 386 variants across 152 key cancer genes. This includes 249 variants with a COSMIC ID and 30 INDELs (24 deletions and 6 insertions, ranging from 2-16 base pairs). Variants are present between 1-100% allelic frequency (AF), with 52 variants present at ≤ 20% AF for LOD determination of your assay.

Every batch of OncoSpan DNA has 25 variants confirmed by ddPCR, in addition to being fully exome sequenced by GATC-Eurofins at 500x coverage using Agilent SureSelect Human All Exon V6 kit and Illumina sequencing to ISO 17025. This provides you with an accurate and reliable truth set for comparison to your assay’s performance. You will receive a password upon ordering to download the raw NGS data for your batch (bam, bed and vcf files) via our File Transfer Protocol (FTP) portal. Alternatively, for your convenience we have also listed the 386 high confidence variants in an easy-to-access excel document that can be downloaded from the “More Information” button above.

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Technical Data

Genes Covered: ABL1, AKT1, AKT2, ALK, APC, AR, ARID1A, ATR, ATRX, AXL, BARD1, BCL6, BLM, BMPR1A, BRAF, BRCA1, BRCA2, BTK, BUB1B, CARD11, CCND1, CCND3, CCNE1, CD79B, CDH1, CDK12, CDK4, CEP57, CFH, CREBBP, CSF1R, CTNNB1, DDR2, DIS3L2, DNMT3A, EGFR, EML4, EP300, EPCAM, ERBB2, ERBB3, ERCC1, ERCC2, ERCC4, ERCC5, ERG, ETS1, ETV4, EWSR1, EXT1, FANCA, FANCD2, FANCE, FANCG, FANCI, FANCM, FBXW7, FGF10, FGF2, FGF3, FGF6, FGFR1, FGFR3, FLCN, FLI1, FLT1, FLT3, FZR1, GATA2, GATA3, GEN1, GNA11, GNAS, HNF1A, HRAS, IDH1, IDH2, JAK1, JAK2, JAK3, KDR, KIT, KRAS, LDLR, MAGI1, MAP2K1, MAP2K2, MAX, MDM4, MED12, MET, MLH1, MLLT3, MMAB, MRE11, MSH2, MSH3, MSH6, MTOR, NBN, NF1, NFE2L2, MOTCH1, NOTCH2, NOTCH3, NRAS, NRG1, NTRK1, NTRK3, PDGFRA, PDGFRB, PIK3CA, PIK3CD, PIK3CG, PIK3R1, PMS2, PPARG, PPP2R2A, PRKAR1A, PROC, PTCH1, PTPN11, RAD51B, RAD54L, RAF1, RB1, RBM45, RECQL4, RET, RHBDF2, ROS1, RPS6KB1, SDHB, SF3B1, SF3B2, SLTM, SLX4, SMARCB1, SMO, SMOX, STK11, TERT, TET2, TFRC, TP53, TP53BP1, TSC1, TSC2, WRN, XPA, XPC, ZNF395

Cosmics: 249

Allelic Frequencies: 1% - 92.5% for ddPCR-verified variants

Buffer: Tris-EDTA (10mM Tris-HCl, 1mM EDTA), pH 8.1

Unit Size: 1µg

Concentration: 50ng/µl

General Information

Storage: 4˚C

Expiry: 36 months from the date of manufacture

Quality Control

Allelic Frequency: Droplet Digital PCR™
Genotype: Next Generation Sequencing
Quality: Agarose gel electrophoresis
Quantification: Spectrophotometry (A260)