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Producenci

One.Step RT-qPCR Probe Kit with ROX (2X concentrated)

nr kat.: 105-510
Opakowanie: 100 x 25 μl rxns
Cena brutto: do wyceny
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Producent: GeneOn
nr kat.: 105-510

Opis

Features:

  • The kit contains all reagents required for RT-qPCR in a set to ensure fast and easy preparation with a minimum of pipetting steps. Just add template, primers and the dual labeled fluorescent probe.
  • The One.Step RT-PCR Kit is highly sensitive: less than 10 pg to 100 ng total RNA or < 1 pg to 20 ng poly(A) RNA (mRNA) can be detected when using highly expressed transcripts.

Description:
The enzyme mix is based on a new formulated reverse transcriptase, a Hot-Start Polymerase and RNase Inhibitor. The set is providing increased specificity, high cDNA yield and improved efficiency for highly structured and long cDNA fragments. The reaction buffer includes extrapure dNTPs; ROX and reaction enhancers. The basis is a Real-time PCR with dual labeled probes and multiplexing capacity.

Principal:
In the RT-step the reverse transcriptase synthesizes single-stranded DNA molecules (cDNA) complementary to the RNA template. The hot-start polymerase activity is blocked.
In the first cycle of the PCR step the Hot-Start DNA polymerase activity is switched on and it synthesizes DNA molecules complementary to the cDNA, thus generating a double-stranded DNA template. The kit is optimized for all real-time PCR cyclers who are compatible with the evaluation of the ROX reference signal.

The One.step Realtime RT-PCR Master Mix from GeneON offers a tremendous convenience when applied to analysis of targets from multiple samples of RNA and minimizes the risk of contaminations.
 
Platforms: The Kit is suitable for all block-based Thermocycler. Stringent Quality Tests on ABI StepOne plus PCR Cycler

Components of Maximo.OneStep RT-qPCR Master Mix (for probes):

  • Enzyme-mix: HotStart Taq Polymerase, Reverse Transcriptase, RNase Inhibitor and enhancers, Reaction buffer containing extra pure dNTPs and ROX
  • RNase-free water

Protocols / Manuals
The datasheet includes detailed protocols for:

1.) RT-PCR assay without sample denaturation (as a standard RNA/primer combinations)

2.) RT-PCR assay with sample denaturation (RNA/primer with a high degree of secondary structure)

2.a) Preparation of RNA / Primer Mix
2.b) Denaturation and primer annealing
2.c) Preparation of the RT-PCR Mix


3.) Reverse Transcription and thermal cycling

Storage: @ -20°C, avoid frequent thawing and freezing, store all components with ROX in the dark

For optimal results it is recommended to make an individually optimization for each RNA / primer pair.

Manufactured and quality-controlled in accordance with ISO 9001:2000.

Dane techniczne

Opakowanie 100 x 25 μl rxns

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