PageRuler™ Prestained Protein Ladder

Sprawdź także MWP03

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Wcześniejszy numer katalogowy: SM0671

Thermo Scientific PageRuler Prestained Protein Ladder is a mixture of ten (10) blue-, orange- and green-stained proteins (10 to 180 kDa) for use as size standards in protein electrophoresis (SDS-PAGE) and Western blotting.

Features of PageRuler Prestained Protein Ladder:

• Size range—10 proteins spanning 10 to 180 kDa
• Ready-to-use—supplied in a loading buffer for direct loading on gels; no need to boil
• Sharp bands—color-coded bands of similar intensity for easy visualization
• Quality tested—each lot evaluated by SDS-PAGE and Western blotting
• Two reference bands—orange at 70kDa and green at 10kDa
• Membrane-compatible—colored bands transfer to membranes for Western blotting

This prestained protein MW marker is designed for monitoring the progress of SDS-polyacrylamide gel electrophoresis, for assessing transfer efficiency onto PVDF, nylon and nitrocellulose membranes, and for estimating the approximate size of separated proteins that have been made visible with gel stains or Western blot detection reagents. The ladder contains one orange reference band at 70kDa and one green band at 10kDa.

Recommendations for Loading
1. Thaw the ladder at room temperature for a few minutes to dissolve precipitated solids. Do not boil!
2. Mix gently, but thoroughly, to ensure the solution is homogeneous.
3. Load the following volumes of the ladder on an SDS-polyacrylamide gel:
– 5 μl per well for mini gel,
– 10 μl per well for large gel.
Use the same volumes for Western blotting.
The loading volumes listed above are recommended for gels with a thickness of 0.75-1.0 mm. The loading volume should be doubled for 1.5 mm thick gels.

Important Notes
• Prestained proteins can have different mobilities in various SDS-PAGE-buffer systems. However, they are suitable for approximate molecular weight determination when calibrated against unstained standards in the same system. See the table provided for migration patterns in different electrophoresis conditions.
• In low-percentage gels (< 10%), the low-molecular w eight proteins in the ladder may migrate with the dye front.
• PageRuler Prestained Protein Ladder can be used in Western blotting with all common membranes: PVDF, nylon and nitrocellulose.
• Longer transfer times or higher transfer voltages may be required for Western blotting of large (>100 kDa) proteins.

Includes:
• Dye-stained proteins in 62.5 mM Tris-H3PO4 (pH 7.5 at 25°C), 1 mM EDTA, 2% SDS, 10 mM DTT, 1 mM NaN3 and 33% glycerol.