PageRuler™ Unstained Low Range Protein Ladder

Sprawdź także: MB21401

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Thermo Scientific PageRuler Unstained Low Range Protein Ladder is a mixture of seven (7) purified proteins and peptides (3.4 to 100kDa) for use as size standards in peptide and small-protein gel electrophoresis.

Features of PageRuler Unstained Low Range Protein Ladder:

• Size range—seven proteins and one peptide spanning 3.4 to 100kDa
• Ready-to-use—supplied in a loading buffer for direct loading on gels; no need to boil
• Sharp bands—excellent accuracy for detection in with coomassie or silver stains
• Reference bands—25kDa band is stronger than the others for easy orientation
• Tagged—each protein in the ladder contains an integral Strep-tag™ II Sequence
• Quality tested—each lot evaluated by SDS-PAGE and Western blotting

The protein MW markers in this ladder resolve into clearly identifiable sharp bands when analyzed by SDS-PAGE and stained with coomassie dye or silver. The 100kDa protein band indicates the approximate position of large proteins and the 25kDa band is of greater intensity and serves as a reference band. Proteins can be detected on Western blots by staining with Ponceau S, coomassie blue or other protein stains. In addition, the ladder proteins (except for the 5 and 3.4kDa peptides) contain an integral Step-tag™ II Sequence and may be detected on Western blots using Strep-Tactin™ Conjugates. The PageRuler Unstained Broad Range Protein Ladder is supplied ready-to-use: no heating, dilution or addition of a reducing agent is required before use.

Includes:
Proteins in 62.5 mM Tris-H3PO4 (pH 7.5 at 25°C), 1 mM EDTA, 2% SDS, 100 mM DTT, 1 mM NaN3, 0.01% bromophenol blue and 33% glycerol

Applications:
Accurate protein sizing on SDS-polyacrylamide gels and Western blots.