PageRuler™ Unstained Low Range Protein Ladder
Opis
Sprawdź także: MB21401
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Thermo Scientific PageRuler Unstained Low Range Protein Ladder is a mixture of seven (7) purified proteins and peptides (3.4 to 100kDa) for use as size standards in peptide and small-protein gel electrophoresis.
Features of PageRuler Unstained Low Range Protein Ladder:
- Size range—seven proteins and one peptide spanning 3.4 to 100kDa
- Ready-to-use—supplied in a loading buffer for direct loading on gels; no need to boil
- Sharp bands—excellent accuracy for detection in with coomassie or silver stains
- Reference bands—25kDa band is stronger than the others for easy orientation
- Tagged—each protein in the ladder contains an integral Strep-tag™ II Sequence
- Quality tested—each lot evaluated by SDS-PAGE and Western blotting
The protein MW markers in this ladder resolve into clearly identifiable sharp bands when analyzed by SDS-PAGE and stained with coomassie dye or silver. The 100kDa protein band indicates the approximate position of large proteins and the 25kDa band is of greater intensity and serves as a reference band. Proteins can be detected on Western blots by staining with Ponceau S, coomassie blue or other protein stains. In addition, the ladder proteins (except for the 5 and 3.4kDa peptides) contain an integral Step-tag™ II Sequence and may be detected on Western blots using Strep-Tactin™ Conjugates. The PageRuler Unstained Broad Range Protein Ladder is supplied ready-to-use: no heating, dilution or addition of a reducing agent is required before use.
Includes:
Proteins in 62.5 mM Tris-H3PO4 (pH 7.5 at 25°C), 1 mM EDTA, 2% SDS, 100 mM DTT, 1 mM NaN3, 0.01% bromophenol blue and 33% glycerol
Applications:
Accurate protein sizing on SDS-polyacrylamide gels and Western blots.
Dane techniczne
Opakowanie | 2x250 µl |