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Phage λ-DNA

nr kat.: 301-025
Opakowanie: 500 µg
Cena brutto: 492,12 zł 492.12
Cena netto: 400,10 zł
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Producent: GeneOn
nr kat.: 301-025

Opis

λ DNA is used as one of the substrates in restriction enzymes research and for testing of restriction endonucleases activity.

Description/Preparation:
The double stranded DNA is isolated from bacteriophage lambda (cl857 ind1 Sam7). The molecular weight is 31.5 x 106 daltons and it is 48,502 base pairs in length.
The phage is isolated from the heat inducible lysogen E. coli λ cl857 S7 by gel filtration. The DNA is isolated from the purified phage by phenol/chloroform extraction and dialyzed against 10 mM Tris-HCl (pH 7.4) and 1 mM EDTA.

Concentration:    0.2-0.5 µg/µl

Storage Buffer:  10 mM Tris-HCl (pH 7.4), 1 mM EDTA.

Quality control: Gel analysis for purity. EcoRI and HindIII fragmentation patterns.
 
Transportation: Shipped on blue ice or room temperature

Storage: at -20°C for 24 months

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