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Protease Inhibitor Cocktail Kit

nr kat.: 0215883701
Opakowanie: 1 kit
Cena brutto: do wyceny
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Producent: MP Biomedicals
nr kat.: 0215883701

Opis

Product Description
Protease Inhibitor Cocktail Kit is a broad spectrum protease inhibitor cocktail which covers both endopeptidases and exopeptidases, non-specific and specific. The cocktail consists of four major protease inhibitors: AEBSF, Sodium EDTA, Leupeptin and Pepstain A.

Preparation Method
Place the contents of all four vials in one volumetric flask or other suitable container. Add water or buffer of choice to 100 mL. More concentrated solutions can be prepared as desired; however, leupeptin and pepstatin A will create more of a suspension at concentrations other than the suggested amount. The prepared solution is stable for 60-90 days when stored at 5 °C. Do not freeze the prepared solution, and avoid repeated freeze-thaw cycles.

Applications
Protease Inhibitor Cocktail Kit contains inhibitors with a broad specificity for serine, cysteine, and acid proteases, and aminopeptidases. It has been tested on a wide variety of cells and tissue extracts to isolate/maintain intact proteins without degradation.

Biochem/physiol Actions
Proteases are enzymes which direct their action upon proteins, cleaving their targets into smaller fragments consisting of polypeptides, peptides and amino acids. There are a myriad of proteases active against practically all proteins. Some proteases are quite specific, while others cleave many different proteins. MP Protease Inhibitor Cocktail covers a broad spectrum of protese inhibitors.

Recommended Use
One mL of the cocktail solution is recommended for the inhibition of proteases equivalent to 1 mg of USP pancreatin. One kit is recommended for the inhibition of proteases present in a maximum of 20 g of cell extract. Since not all organisms contain the same amount of endogenous proteases it may sometimes be necessary to increase the concentration of inhibitors. Typical use is 1 mL of cocktail per 1 mL of sample.

Typical Working Concentration
Typical working concentration for 100 mL of inhibitor cocktail:

  • AEBSF Hydrochloride:50-400 mg/mL;
  • EDTA disodium dihydrate:0.2-25 mg/mL;
  • Leupeptin Hemisulfate: 0.5-1 mg/mL;
  • Pepstatin A: 0.5-1 mg/mL


Usage Statement
Research Use Only (RUO). Ready for CE IVD labeling of clinical applications.

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