Quantitative Multiplex Reference Standard (FFPE)

Format
FFPE

Description
The Quantitative Multiplex FFPE Reference Standard is a highly-characterized, biologically-relevant quality control material used to assess the performance of NGS assays that detect somatic mutations.

With this product you are able to:

• Evaluation of workflow integrity from pre-analytical DNA extraction to post-analytical bioinformatics
• Analyze the robustness of your workflow with multiple formats (DNA , FFPE and Formalin-Compromised DNA)
• Optimize and validate new cancer panels and routinely monitor the performance of your assay

Next generation sequencing (NGS) platforms give researchers much greater power when profiling tumor samples. However, the use of NGS presents challenges surrounding sample variability, platform bias, and potential failure to detect biomarkers. Horizon’s standards are appropriate for any NGS library preparation including whole-genome, whole-exome, custom capture and targeted amplicon panels. Horizon has developed this Quality-Seq (Q-Seq) NGS Reference Standard range to support the development and continued validation of Next Generation Sequencing platforms.

The Quantitative Multiplex Reference Standard (QMRS) portfolio covers multiple endogenous SNPs, insertions and deletions. The QMRS includes 11 mutations at 0.8-24.5% allelic frequency in genomic DNA, FFPE and Formalin-Compromised DNA format. The multiple formats of the QMRS allow you to validate both the pre-analytical and analytical portion of your workflow using an isogenic reference standard. T

he FFPE format QMRS allows you to evaluate and optimize DNA extraction protocols without wasting precious patient samples. With the Formalin-Compromised format, the robustness of your NGS workflow may be tested to ensure that your library protocol and informatics pipeline can accommodate these highly degraded samples.

The QMRS standard is our most popular multiplex standard as it provides both a breadth of formats, but also a large range of allelic frequencies and onco-relevant mutants.

Technical Data

Genes Covered: BRAF, KIT, EGFR, KRAS, NRAS, PIK3CA

Cosmics: 1,500+

Fixation Method: 10% Formalin

Section Size: 15µm or 20µm

Cell Density: 3 x 10⁸ cells per block. Approx. 3.5 x 10⁵ cells per section

Expected DNA Yield: > 400 ng DNA using Promega Maxwell LEV Plus Extraction kit

Unit Size: 1 FFPE Section

Extractable DNA: ≥ 400 ng DNA per section using Promega Maxwell LEV Plus FFPE DNA Extraction kit

Intended Use: For routine performance monitoring (Research Use Only)

General Information

Allelic Frequency: 1% - 24.5%  

Storage: 4˚C

Expiry: 48 months from the date of manufacture

Cell Line Background: HCT116/RKO/SW48

Quality Control

Genotype: Sanger sequencing of locus specific PCR
Quality: Agarose gel electrophoresis
Quantification: Quantifluor™