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RiboEx™ LS Total RNA mini

nr kat.: 302-002
Opakowanie: 2x100 ml
Cena brutto: do wyceny
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Producent: GeneALL
nr kat.: 302-002

Opis

RiboEx LS is a complete kit with ready-to-use reagents for the isolation of total RNA from various liquid samples.

RiboEx™ LS is a concentrated form of RiboEx™ and this allows that liquid samples can be processed more easily with it, while RiboEx™ is more suitable for solid samples and pelleted cells. RiboEx™ LS is a mono-phasic solution containing phenol and guanidine salt, which rapidly lyse cells and inactivates nucleases. Addition of chloroform b

rings about a separation of the homogenate in aqueous and organic phases. RNA locates in the aqueous phase while DNA and protein remain in the interphase and organic phases. The aqueous phase including RNA is mixed with isopropanol and the RNA which is precipitated by centrifuging.

The purified total RNA is suitable for RT-PCR, northern blotting, dot blotting, in vitro translation, molecular cloning, realtime PCR, RNase protection assays and other analytical procedures.


Features and Benefits

  • Format: Monophase solution type
  • Sample size: Up to 0.25 ml liquid sample / 0.75 ml RiboExTM LS
                            Up to 100 mg tissue / 0.75 ml RiboExTM LS
  • Preparation time: 50 ~ 65 minutes
  • Typical yield: Up to 30 ㎍ / 1 x 106 cultured cells
                            Up to 10 ㎍ / 1 mg tissue
  • High purity: A260/A230 > 2.0, A260/A280 > 1.8
  • Accurate and consistence yield
  • Ready for use in RT-PCR, northern blotting, dot blotting, in vitro translation, molecular cloning, real-time  PCR, RNase protection assays and other analytical procedures

 

Dane techniczne

Opakowanie 2x100 ml

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