Produkty
Producenci
S1 Nuclease
nr kat.:
EN0321
Opakowanie:
10000 U
Producent:
nr kat.:
EN0321
Opis
Concentration: 100 U/µL
Thermo Scientific S1 Nuclease degrades single-stranded nucleic acids, releasing 5'-phosphoryl mono- or oligonucleotides . It is five times more active on DNA than on RNA. S1 Nuclease also cleaves dsDNA at the single-stranded region caused by a nick, gap, mismatch or loop. S1 Nuclease exhibits 3'-phosphomonoesterase activity.
The enzyme is a glycoprotein with carbohydrate content of 18%.
Applications
Removal of single-stranded overhangs of DNA fragments
- S1 transcript mapping
- Cleavage of hairpin loops
- Creation of unidirectional deletions in DNA fragments in conjunction with Exonuclease III
Note
S1 Nuclease can introduce breaks into double-stranded DNA, RNA and DNA/RNA hybrids at high enzyme and low salt concentrations.
Dane techniczne
| Opakowanie | 10000 U |