SPINeasy RNA Kit for Bacteria (with Lysing Matrix)

Quick and convenient purification of total RNA from gram-positive and gram-negative bacteria using spin column technology.

SPINeasy RNA Kit for Bacteria is a silica-membrane spin-column kit that enables quick and convenient purification of total RNA from gram-positive and gram-negative bacteria. Included in the kit is our specially formulated RNASS solution that stabilizes and protects RNA in bacteria samples. The use of Lysis Buffer R and Lysing Matrix B in combination with FastPrep® instruments from MP Biomedicals enables highly efficient lysis of bacterial samples within seconds. With a simple workflow, the kit allows multiple samples to be processed simultaneously, without the use of toxic substances such as phenol and chloroform. Total RNA of high quality and integrity can be purified typically within 40 minutes and is immediately available for RT-PCR and other downstream applications.

Components:

• 50x Lysing Matrix B,
• 15ml RNASS,
• 60ml Lysis Buffer R,
• 6ml Wash Buffer R,
• 10ml Nuclease-free water,
• 1 vial DNase I,
• 5ml DNase I Buffer,
• 50x Column R with collection tube