Sirius 2 Touch-0 Single Tube Luminometer, W/O reagent injector, LB 9526

High performance luminescence detection
The Sirius 2 detects less than 0.5 zmol of firefly luciferase (1 amol ATP/tube). Light collection is maximized through the close proximity of the sample to the detector and the use of optimized light reflectors surrounding the sample vial. The detector is operated in photon counting mode, which ensures a very low background signal for excellent signal to noise ratio and the highest linearity.

Outstanding flexibility
The sample chamber is designed for performance and flexibility: from 35 mm petri dishes to microfuge format to 20 mL vials, just pick the sample format that fits your application best. The optional sample holders enable accurate injection into both, microcentrifuge tubes and standard luminescence tubes.

The system is compatible with a variety of sample formats:

•     Standard 12 mm luminescence tubes (55 and 75 mm)
•     Microcentrifuge tubes (1.5 and 2 mL)
•     Scintillation vials (up to 20 mL)
•     35 mm Petri dishes

Intuitive touchscreen operation
The touchscreen-driven software is very intuitive and operated by touch gestures. The easy-to-use software provides advanced features, such as handling of replicates, dual assay calculations, and display of graphics.
Data can be downloaded easily onto a USB stick or printed. The results can be exported for further calculation in Excel or other programs.
 
Automatic quickstart
Closing of the sample drawer automatically initiates the measurement. There´s no need to press any key. The automatic light shutter protects the detector from ambient light. This makes the system ready for measurement from the moment the door is closed. This means that there is no need to set a long delay before the measurement. This is necessary with most other tube luminometers so that the background can return to the initial level after the door has been opened.

Flexible injector solutions
The system is available with up to two syringe-type automatic reagent injectors. The injection volume range is 20–500 μL. This very broad volume range allows the Sirius 2 to adapt to any assay using injectors. Ready-to go sample holders for precise injection are available for 1.5 ml and 2 ml microfuge tubes, as well as for 55 mm and 75 mm, 12 mm diameter tubes (standard format for most luminescence assays).

Technical Specification

Measurement
Detectors: Low-noise photomultiplier tube in single photon counting mode
Detection technologies:    Luminescence
Measurement modes: Endpoint, Kinetics, Multiple Kinetics
Spectral range luminescence: 300-600 nm

Injectors
Injectors: Up to 2 syringe injectors
Injector volume: 20-500 µL    

Tube formats    
Luminescence tubes, 12 x 55 mm
Luminescence tubes, 12 x 75 mm
Microcentrifuge tubes, 1.5 and 2 mL
Scintillation vials (up to 20 mL)
35 mm Petri dishes

Performance
Luminescence sensitivity: <1 amol ATP/tube (ATP HS assay)
Dynamic range: 6 orders of magnitude

Operation
Interface:    

•     Windows software, connected to computer by USB
•     Optional integrated computer with built-in software

Display (integrated computer): 7” capacitive touch, gesture control, 480 x 800 pixels

External interfaces (integrated computer): 2x USB 2.0, 1x Ethernet (RJ-45)

Printer interface (integrated computer): Network or USB printing (PCL6 or Postscript 3)

Data export: CSV (Excel), PDF, HTML formats

Available protocols:    

•     PC software: Quick Measurement included; additional options (purchased separately): Single Assay, Dual Assay, Single Kinetics, Multiple Kinetics, Cut-Off
•     Built-in computer: Quick Measurement, Single Assay, Dual Assay

Applications

•     Reporter gene assays
•     Luminescent immunoassays
•     ATP quantification
•     Caspase assays
•     Kinase assays
•     Toxicity and mutagenicity of water samples