Speedy NZYProof DNA Polymerase

Wcześniej MB10902 NZYSpeedy Proof DNA Polymerase

Description: Speedy NZYProof DNA polymerase is a recombinant thermostable DNA polymerase purified from Escherichia coli that combines high fidelity and fast polymerization capacities. Speedy NZYProof DNA polymerase possesses 3´→5´ exonuclease proofreading activity that enables the polymerase amplifying DNA with increased accuracy and yield. The error rate of the enzyme is similar to that of Pfu and Kod DNA polymerases (the order of magnitude is between 10^-5 and 10^-6), which is significantly lower than the error rate of Taq DNA polymerases. Speedy NZYProof DNA polymerase is highly efficient in the amplification of DNA fragments up to 3 kb in a short reaction period. Only 15 seconds are required for the successful synthesis of 1 kb size DNA. Speedy NZYProof DNA polymerase generates blunt-ended polymerase chain reaction (PCR) products that are suitable for cloning with NZYTech´s NZY-blunt PCR cloning kit (MB121).

Features:
– Fast amplification (15 sec/kb)
– Proofreading activity

Applications
– High-fidelity PCR
– Fast PCR
– Generation of products for blunt-end cloning

Successful amplification with limited extension time and proofreading activity:
Three different DNA fragments of various sizes and from different origins were amplified using a limited extension period of 15 sec/kb and 2.5 U of Speedy NZYProof DNA polymerase.

Amplification of nucleic acids from different DNA templates using Speedy NZYProof DNA polymerase. Lane 1: 350 bp PCR product (sheep genomic DNA, 120 ng). Lane 2: 1 kb PCR product (E. coli genomic DNA, 100 ng). Lane 3: 2 kb PCR product (plasmid DNA, 10 ng). Lane M: NZYDNA Ladder III.

Specifications:

Product length:	0-3 kb
Hot-start like capacity:	no
Speed:	15 sec/kb
Product overhang:	blunt
Storage conditions:	Store at -20 ºC
Shipping conditions:	Shipped at 4 ºC to dry ice

Components:
– Speedy NZYProof DNA polymerase (2.5 U/μL)
– Reaction buffer (10x)
– MgCl₂ (50 mM)