SphI
Opis
5’...GCATG↓C...3’
3’...C↓GTACG...5’
SphI is a Restriction Endonuclease purified from an Escherichia coli strain that carries the SphI gene from Streptomyces phaeochromogenes. SphI activity is not affected by dam methylation, dcm methylation or CpG methylation. NZYTech’s SphI is an enzyme that acts at 37 °C.
Isoschizomers: BbuI, PaeI, SpaHI
Concentration: 10 U/μL
Unit definition: One unit is defined as the amount of this enzyme required to digest completely 1 μg of plasmid DNA in 50 μL of the reaction mixture at 25 °C for one hour.
Incubation temperature: 37 °C for one hour
Heat inactivation: 65 °C for 20 minutes
Reaction Buffer: NZYTech provides SphI with a specific buffer, NZYBuffer A. For double digestions please use 10x NZYBuffer U (MB110, not provided) but star activity may occur.
Activity in NZYTech Buffers:
NZYBuffer A: 100%
NZYBuffer B: 100%
NZYBuffer C: (80)%
NZYBuffer U: (100)%
() star activity is detected
Dane techniczne
Opakowanie | op. |