Supreme NZYLong 2x Colourless Master Mix

Description: Supreme NZYLong 2× Colourless Master Mix is a premixed ready-to-use solution containing Supreme NZYLong DNA polymerase (MB331), an engineered version of NZYLong DNA polymerase designed to amplify longer PCR products, generally of 25 kb and beyond, that displays a higher specificity provided by its hot-start-like PCR capacity. This feature is achieved by a novel hot-start technology, which inhibits both polymerase and 3’→5’ exonuclease activities and thus avoids extension of non-specifically annealed primers or primer-dimers, as well as degradation of primers and template DNA during PCR reaction setup. The functional activity of the enzyme is restored during a short 5-minute incubation step at 94 °C. The increased processivity of Supreme NZYLong DNA polymerase combined with the hot-start-like PCR capacity results in higher specificity, sensitivity and yield during the amplification of long nucleic acids. The master mix contains dNTPs, reaction buffer and additives at optimal concentrations for the efficient amplification of a wide range of DNA templates. MgCl₂ final concentration is 2.5 mM, allowing the implementation of a variety of PCR protocols.

Supreme NZYLong DNA polymerase generates a mixture of A-overhang-ended (predominantly) and blunt-ended PCR products, being suitable for cloning with NZYTech´s TA PCR cloning kits (MB053 or MB137).

Features:
– Convenient ready-to-use PCR master mix
– Amplifies long DNA fragments
– High specificity: Eliminates nonspecific amplifications and minimizes primer-dimers
– Higher fidelity than NZYTaq DNA polymerase

Applications:
– Long-Range PCR
– Colony PCR
– Generation of products for TA cloning

Specifications:

Components:
Supreme NZYLong 2× Colourless Master Mix (0.2 U/μL)