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Supreme NZYLong 2x Green Master Mix

nr kat.: MB33301
Opakowanie: 100 react
Cena brutto: 407,99 zł 407.99
Cena netto: 331,70 zł
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Producent: NZYTech
nr kat.: MB33301

Opis

Description: Supreme NZYLong 2× Green Master Mix is a premixed ready-to-use solution containing Supreme NZYLong DNA polymerase (MB331), which is an optimised DNA polymerase designed to amplify target DNA sequences up to 20 kb in size with high specificity provided by the inherent hot-start-like PCR capacity. The enzyme is inactive at room temperature, avoiding extension of non-specifically annealed primers or primer-dimers. The functional activity is restored during a short 5-minute incubation step at 95 °C. In addition, the master mix contains dNTPs and reaction buffer at optimal concentrations for efficient amplification of a wide range of DNA templates. MgCl2 final concentration is 2.5 mM, allowing the implementation of a comprehensive variety of PCR protocols. In addition, reactions assembled with Supreme NZYLong 2× Green Master Mix may be directly loaded onto agarose gels. There are two dyes (blue and yellow) in the mix that allow monitoring the progress of electrophoresis.

NZYLong DNA polymerase generates a mixture of A-overhang-ended (predominantly) and blunt-ended PCR products, being suitable for cloning with NZYTech´s TA PCR cloning kits (MB053 or MB137).

Features:
– Convenient ready-to-use PCR master mix
– Direct Gel Loading
– Amplifies long DNA fragments
– High specificity: Eliminates nonspecific amplifications and minimizes primer-dimers
– Higher fidelity than NZYTaq DNA polymerase

Applications:
– Long-Range PCR
– Colony PCR
– Generation of products for TA cloning

Specifications:

Product length: 0-20 kb
Hot-start-like capacity: no
Extension time: 60 sec/kb at 68 °C
3´→5´ activity: no
Product overhang: 3’-A
Storage conditions: Store at -20 ºC
Shipping conditions: Shipped at 4 ºC to dry ice

Components:
Supreme NZYLong 2× Green Master Mix (0.2 U/μL)

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