Description: Supreme NZYLong DNA polymerase is an optimised DNA polymerase designed to amplify target DNA sequences up to 20 kb in size with high specificity provided by its hot-start-like PCR capacity. The enzyme is inactive at room temperature, avoiding extension of non-specifically annealed primers or primer-dimers and thus providing a higher specificity of DNA amplification. The functional activity of the enzyme is restored during a short 5-minute incubation step at 95 °C. Supreme NZYLong DNA polymerase displays a higher fidelity than conventional Taq DNA polymerases. The enzyme generates a mixture of A-overhang-ended (predominantly) and blunt-ended PCR products, being suitable for cloning with NZYTech´s TA PCR cloning kits (MB053 or MB137).
Features:
– Amplifies long DNA fragments
– High specificity: Eliminates nonspecific amplifications and minimizes primer-dimers
– Higher fidelity than NZYTaq DNA polymerase
Applications:
– Long-Range PCR
– Generation of products for TA cloning
Specifications:
Product length: | 0-20 kb |
Hot-start like capacity: | no |
Extension time: | 60 sec/kb at 68 °C |
Product overhang: | mixed |
Available as Mixes: | Supreme NZYLong 2x Green Master Mix (MB333); Supreme NZYLong 2x Colourless Master Mix (MB334) |
Storage conditions: | Store at -20 ºC |
Shipping conditions: | Shipped at 4 ºC to dry ice |
Components:
– Supreme NZYLong DNA polymerase (5 U/μL)
– Reaction buffer (10x)
– Stablilizer Solution (5x)