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Producenci

Supreme NZYProof 2x Green Master Mix

nr kat.: MB28502
Opakowanie: 200 react.
Cena brutto: 869,73 zł 869.73
Cena netto: 707,10 zł
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Producent: NZYTech
nr kat.: MB28502

Opis

Description: Supreme NZYProof 2× Green Master Mix is a premixed ready-to-use solution containing Supreme NZYProof DNA polymerase (MB283), an engineered highly accurate, fast and sensitive variant of NZYProof DNA polymerase displaying a hot-start like PCR capacity. This feature is achieved by a novel hot-start technology, which inhibits both polymerase and 3’→5’ exonuclease activities and thus avoids extension of non-specifically annealed primers or primer-dimers, as well as the degradation of primers and template DNA during PCR reaction setup. Thus, Supreme NZYProof DNA polymerase generates higher specificity, sensitivity and yield during the accurate amplification of DNA. The master mix contains dNTPs, reaction buffer and additives at optimal concentrations for the efficient amplification of DNA templates by PCR. Mg2+ final concentration is 1.5 mM, allowing the implementation of a variety of PCR protocols. In addition, reactions assembled with Supreme NZYProof 2× Green Master Mix may be directly loaded onto agarose gels. Supreme NZYProof 2× Green Master Mix contains two dyes (blue and yellow) that allow monitoring the progress of electrophoresis.

Supreme NZYProof DNA polymerase possesses 3´→5´ exonuclease proofreading activity. Resulting PCR products have blunt-ends and are suitable for cloning with NZYTech´s NZY-blunt PCR cloning kit (MB121).

Features:
– Convenient ready-to-use PCR master mix
– Direct Gel Loading
– Hot-start like capacity
– Proofreading activity

Applications:
– High-fidelity PCR
– Hot-start PCR
– Routine PCR
– Generation of products for blunt-end cloning

Increased sensitivity of Supreme NZYProof 2x Green Master Mix
Superior sensitivity of Supreme NZYProof DNA polymerase present in the 2x Green Master Mix allows amplification using few amounts of DNA template.

 

Specifications:

Product length: 0-10 kb
Hot-start like capacity: yes
Extension time: 15-30 sec/kb at 72 °C
3´→5´ activity: yes
Product overhang: blunt
Storage conditions: Store at -20 ºC
Shipping conditions: Shipped at 4 ºC to dry ice

Components:
Supreme NZYProof 2× Green Master Mix (0.2 U/μL)

Dane techniczne

Opakowanie 200 react.

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