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Supreme NZYProof DNA polymerase

Cena brutto: 369,14 zł
Cena netto: 341,80 zł
szt.
Opakowanie:
125 U
Kod produktu:

MB28301

Producent:
Pliki do pobrania:

Features:
– High sensitivity
– Eliminates nonspecific amplifications and minimizes primer-dimers
– High processivity/ Fast amplification (15-30 sec/kb)
Proofreading activity 50X than regular Taq!

Description: Supreme NZYProof DNA polymerase contains a recombinant modified form of NZYProof DNA polymerase displaying a hot-start like PCR capacity. This feature is achieved by a novel hot-start technology, which inhibits both polymerase and 3’→5’ exonuclease activities of the enzyme and thus avoids extension of non-specifically annealed primers or primer-dimers, as well as degradation of primers and template DNA during PCR reaction setup. Supreme NZYProof DNA polymerase provides higher specificity, sensitivity and yield of DNA amplification. The functional activity of the enzyme is restored during PCR cycling. In addition, Supreme NZYProof DNA polymerase was engineered for high processivity, thus allowing fast PCR reactions.

This highly robust version of NZYProof DNA polymerase is a broad range enzyme suitable for a variety of applications, including amplification of longer PCR products and site-directed mutagenesis. Supreme NZYProof DNA polymerase possesses 3´→5´ exonuclease proofreading activity that enables the polymerase to amplify DNA with increased accuracy. The error rate of Supreme NZYProof DNA polymerase is similar to that of Pfu and Kod DNA polymerases and significantly lower than the error rate of Taq DNA polymerases. It generates blunt-ended PCR products that are suitable for cloning with NZYTech´s NZY-blunt PCR cloning kit (MB121).

 Applications:
– High-fidelity PCR
– Hot-start PCR
– Amplification of difficult-to-amplify targets (e.g. high GC-contents)
– Site-directed mutagenesis
– Generation of products for blunt-end cloning

High specificity hot-start PCR 
Supreme NZYProof DNA polymerase was engineered to exhibit extreme specificity, enabling accurate amplification of a broad range of DNA templates with different sizes and concentrations. During reaction setup, Supreme NZYProof DNA polymerase is inactivated through a novel hot-start technology, which inhibits both polymerase and 3’-5’ exonuclease activities of the enzyme at room temperature, as well as on ice. Supreme NZYProof DNA polymerase does not require a separate activation step during the PCR cycling, since its activity is immediately restored at high temperatures.

Three E. coli genomic DNA fragments of different lengths (1, 2.5 and 5 kb) were amplified with Supreme NZYProof DNA polymerase (2.5 U). Three 4-fold gDNA dilutions ranging from 40 to 2.5 ng were used as starting template to amplify each DNA fragment. NC: No template control. Lane M: NZYDNA Ladder III (MB044). Results demonstrate amplification in all the conditions tested with high yields and specificity.

 

Specifications:

Product length: 0-10 kb
Hot-start like capacity: yes
Extension time: 15-30 sec/kb at 72 ºC
3´→5´ activity: no
Product overhang: blunt
Available as Mixes: Supreme NZYProof 2x Green Master Mix (MB285); Supreme NZYProof 2x Colourless Master Mix (MB286)
Storage conditions: Store at -20 ºC
Shipping conditions: Shipped at 4 ºC to dry ice

Components:
– Supreme NZYProof DNA polymerase (2.5 U/μL)
– Reaction buffer (5x)

Produkty powiązane

Nazwa produktu / Producent Nr katalogowy Opakowanie Cena brutto  
Supreme NZYProof 2x Colourless Master Mix
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MB28601 100 react.
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Supreme NZYProof 2x Green Master Mix
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MB28501 100 react.
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dNTP NZYMix 10 mM
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MB08604 1 ml
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BL21(DE3)
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MB00601 20 react.
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MB05302 zestaw
534,19 zł
NZYDNA Ladder V (100 bp)
NZYTech
MB06102 500 applic.
604,79 zł

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