Description: Supreme NZYTaq II DNA polymerase is an engineered version of NZYTaq II DNA polymerase displaying a hot-start-like PCR capacity. The enzyme activity at room temperature is limited, avoiding extension of non-specifically annealed primers or primer-dimers and thus providing higher specificity, sensitivity and yield during DNA amplification. The functional activity of the enzyme is restored during a short 5-minute incubation step at 95 °C. Supreme NZYTaq II is provided with 5× Gel Load Reaction Buffer allowing reactions to be loaded directly into gels without the extra adding of loading dye. This Gel Load Reaction Buffer is composed by a blue and yellow dye. The blue dye migrates at the same rate as a 3-5 kb DNA fragment in a 1% (w/v) agarose gel. The yellow dye migrates at a rate faster than primers (<50 bp) in a 1% (v/v) agarose gel. The 5× Gel Load Reaction Buffer is not suitable when direct fluorescent or absorbance readings are required without prior purification of the amplified DNA from PCR. Supreme NZYTaq II DNA polymerase lacks 3´→5´ exonuclease activity. Resulting PCR products have an A-overhang and are suitable for cloning with NZYTech´s NZY-A PCR cloning kit (MB053) or NZY-A Speedy PCR cloning kit (MB137).
Features:
– Suitable for immediate loading onto agarose gels
– High sensitivity and specificity
– Reproducible and convenient (provides the convenience of room temperature reaction set-up)
– Leaves an A-overhang
Applications:
– Powerful PCR
– Amplification of low-copy templates
– High specificity amplifications
– Hot-start PCR
– Generation of products for TA cloning
Specifications:
| Product length: | 0-6 kb |
| Hot-start-like capacity: | yes |
| Extension time: | 15-30 sec/kb at 72 ºC |
| 3´→5´ activity: | no |
| Product overhang: | 3’-A |
| Storage conditions: | Store at -20 ºC |
| Shipping conditions: | Shipped at 4 ºC to dry ice |
Components:
– Supreme NZYTaq II DNA polymerase (5 U/μL)
– Gel Load Reaction buffer (5x)
– MgCl2 Solution (50 mM)