Produkty
Producenci

T4 DNA Polymerase

nr kat.: EP0061
Opakowanie: 100 U
Cena brutto: do wyceny
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Opis

Thermo ScientificT4 DNA Polymerase, a template-dependent DNA polymerase, catalyzes 5'-3' synthesis from primed single-stranded DNA. The enzyme has a 3'-5' exonuclease activity, but lacks 5'-3' exonuclease activity.
 
Highlights

  • Stronger 3'-5' exonuclease activity on single-stranded than on double-stranded DNA and greater (more than 200 times) than DNA
  • polymerase I, E. coli,and Klenow fragment
  • Active in Thermo Scientific restriction enzyme, PCR, RT and T4 DNA Ligase buffers
     

Applications

  • Blunting of DNA ends: fill-in of 5'-overhangs or/and removal of 3'-overhangs
  • Blunting of PCR products with 3'-dA overhangs
  • Synthesis of labeled DNA probes by the replacement reaction
  • Oligonucleotide-directed site-specific mutagenesis
  • Ligation-independent cloning of PCR products

Dane techniczne

Opakowanie 100 U
do góry
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