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T4 DNA ligase

nr kat.: MB00703
Opakowanie: 500 U
Cena brutto: 193,85 zł 193.85
Cena netto: 157,60 zł
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Producent: NZYTech
nr kat.: MB00703

Opis

T4 DNA Ligase is an ultrapure recombinant enzyme purified from Escherichia coli supplied with an optimized 10× Reaction Buffer, which includes ATP. T4 DNA Ligase catalyses the formation of a phosphodiester bond between juxtaposed 5′-phosphoryl and 3′-hydroxyl termini in duplex DNA. It repairs single-strand nicks in duplex DNA and will join both blunt and cohesive-end restriction fragments of duplex DNA or RNA. The enzyme requires ATP as cofactor.

Features:
Free from detectable nonspecific nuclease, endonuclease, RNase and DNase activities
Supplied with an optimized reaction buffer for efficient ligation reactions

Applications:

  • Cloning of both blunt- and sticky (cohesive)-end restriction fragments
  • Cloning of PCR products
  • Joining linkers and adapters to DNA
  • Nick repair
  • Self-circularization of linear DNA


Specifications:

  • Enzyme concentration: 5 U/μL
  • Source: Recombinant E. coli strain
  • Optimal reaction temperature: 16-20 °C
  • Heat inactivation: 65 °C for 10 min
  • Storage conditions: Store at -20 °C
  • Shipping conditions: Shipped at dry ice


Components:

  • T4 DNA Ligase (5 U/μL)
  • Reaction buffer (10x)

EL0011, EL0014, EL0016

Dane techniczne

Opakowanie 500 U

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