Produkty
Producenci

T4 Polynucleotide Kinase

nr kat.: MB00801
Opakowanie: 500 U
Cena brutto: 275,64 zł 275.64
Cena netto: 224,10 zł
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Producent: NZYTech
nr kat.: MB00801

Opis

T4 Polynucleotide Kinase catalyses the transfer of the terminal phosphate of ATP to 5′-hydroxyl termini of polynucleotides such as DNA and RNA, oligonucleotides and 3′-mononucleotides. In the presence of ADP, it will also catalyse the exchange of 5′-terminal phosphate groups and ATP. This enzyme also possesses a 3′-phosphatase and 2′, 3′ cyclic phosphodiesterase activity.

Features

  • Free from detectable exonuclease, endonuclease and ribonuclease activities
  • Supplied with an optimized reaction buffer for efficient activity
  • Supplied with a dilution buffer to optimize levels of incorporation


Applications

  • End-labeling DNA or RNA for use as probes, for sequencing or for DNA-protein footprinting
  • 5´-phosphorylation of oligonucleotides, PCR products, other DNA or RNA to allow subsequent ligation
  • Removal of 3´-phosphoryl groups

EK0031, EK0032


Specifications
Enzyme concentration:    30 U/μL
Source:    Recombinant E. coli strain
Optimal reaction temperature:    37 °C
Heat inactivation:    65 °C for 10 min
Storage conditions:    Store at -20 °C
Shipping conditions:    Shipped at dry ice

Components

  • T4 Polynucleotide Kinase (500 U)
  • Reaction buffer (10x)
  • Dilution buffer


Components

  • Klenow Fragment of DNA Polymerase I (300 U)
  • Reaction buffer (10x)

Dane techniczne

Opakowanie 500 U

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