T4 Polynucleotide Kinase
Opis
T4 Polynucleotide Kinase catalyses the transfer of the terminal phosphate of ATP to 5′-hydroxyl termini of polynucleotides such as DNA and RNA, oligonucleotides and 3′-mononucleotides. In the presence of ADP, it will also catalyse the exchange of 5′-terminal phosphate groups and ATP. This enzyme also possesses a 3′-phosphatase and 2′, 3′ cyclic phosphodiesterase activity.
Features
- Free from detectable exonuclease, endonuclease and ribonuclease activities
- Supplied with an optimized reaction buffer for efficient activity
- Supplied with a dilution buffer to optimize levels of incorporation
Applications
- End-labeling DNA or RNA for use as probes, for sequencing or for DNA-protein footprinting
- 5´-phosphorylation of oligonucleotides, PCR products, other DNA or RNA to allow subsequent ligation
- Removal of 3´-phosphoryl groups
EK0031, EK0032
Specifications
Enzyme concentration: 30 U/μL
Source: Recombinant E. coli strain
Optimal reaction temperature: 37 °C
Heat inactivation: 65 °C for 10 min
Storage conditions: Store at -20 °C
Shipping conditions: Shipped at dry ice
Components
- T4 Polynucleotide Kinase (500 U)
- Reaction buffer (10x)
- Dilution buffer
Components
- Klenow Fragment of DNA Polymerase I (300 U)
- Reaction buffer (10x)
Dane techniczne
Opakowanie | 500 U |