Description: T7 Endonuclease I is an ultrapure recombinant enzyme purified from Escherichia coli supplied with an optimized 10× Reaction Buffer. This endonuclease is the product of phage T7 gene 3. T7 Endonuclease I recognizes and cleaves non-perfectly matched DNA, cruciform DNA structures, Holliday structures or junctions, heteroduplex DNA and more slowly, nicked double stranded DNA. The cleavage site is the first, second or third phosphodiester bond that is 5´ to the mismatch.
Features:
– >95% pure as judged by SDS-PAGE
– Structure-selective enzyme
– Supplied with an optimized reaction buffer for efficient activity
Applications:
– Resolving four-way junction or branched DNA
– Detection or cleavage of heteroduplex and nicked DNA
– Random cleavage of linear DNA for shot-gun cloning
Specifications:
| Enzyme concentration: | 10 U/μL |
| Source: | Recombinant E. coli strain |
| Optimal reaction temperature: | 37 °C |
| Heat inactivation: | 65 °C for 20 min |
| Storage conditions: | Store at -20 °C |
| Shipping conditions: | Shipped at dry ice |
Components:
– T7 Endonuclease I (250 U)
– Reaction buffer (10x)