Produkty
Producenci

T7 RNA Polymerase

nr kat.: MB08003
Opakowanie: 10000 U
Cena brutto: 271,46 zł 271.46
Cena netto: 220,70 zł
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Producent: NZYTech
nr kat.: MB08003

Opis

Description: T7 RNA polymerase is a recombinant enzyme purified from Escherichia coli, for the synthesis of highly specific activity RNA probes, biologically active mRNA and antisense RNA. T7 RNA polymerase is DNA-dependent with strict specificity for its own double-stranded promoter that is not efficiently recognized by SP6 or T3 RNA polymerases. T7 RNA polymerase catalyzes the 5’→3’ synthesis of RNA from ribonucleoside triphosphates on single or double stranded DNA downstream from a T7 promoter. Using circular plasmid DNA as a template will result in heterogeneous transcripts of multiple lengths. T7 RNA polymerase accepts modified nucleotides as substrates for RNA synthesis.

Features:
– Quick and easy production of RNA transcripts from DNA template containing a RNA polymerase promotor
– Convenient and reliable
– Incorporates modified nucleotides as substrates for RNA synthesis

Applications:

– Synthesis of RNA transcripts, biologically active mRNA and antisense RNA
– Production of RNA for in vitro translation and gene expression studies
– Generation of labelled RNA probes

Specifications:

Specificity: Highly promoter-specific. Only transcribes DNA cloned downstream of T7 promoter
Optimal reaction temperature: 37 ºC
Denaturing conditions:  T7 RNA polymerase can be inactivated by adding 20 mM EDTA (pH 8.0) final concentration or by heating at 70 °C for 10 min
Storage conditions: Store at -20 ºC
Shipping conditions: Shipped at 4 ºC to dry ice


Components:
– T7 RNA Polymerase (200 U/μL)
– Reaction Buffer (10x)

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