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Taq DNA Ligase

nr kat.: MB42601
Cena brutto: 249,32 zł 249.32
Cena netto: 202,70 zł
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Producent: NZYTech
nr kat.: MB42601

Opis

Description: Taq DNA Ligase is a NAD+ dependent thermostable DNA ligase that catalyses the formation of a phosphodiester bond between juxtaposed 5 ́ phosphate and 3 ́ hydroxyl termini of two adjacent nucleotides in the context of a double-stranded DNA molecule. The ligation will occur only if the adjacent nucleotides are perfectly paired and have no gaps between them, thus allowing the use of this enzyme as a tool to detect single-base substitutions. As expected from its origin from a thermophilic host, Taq DNA Ligase is active at elevated temperatures (45–65°C). Due to critical differences in substrate specificity, Taq DNA Ligase is not a substitute for T4 DNA Ligase and it is not suitable for cloning applications or adapter ligation/NGS library prep. Taq DNA Ligase is primarily used in molecular diagnostic applications and for direct cloning applications.

Features:
– Thermostable DNA Ligase
– Active at elevated temperatures (45–65°C)
– Selective ligation of nicks in dsDNA
– Taq DNA Ligase is not a substitute for T4 DNA Ligase
– NAD-dependent DNA ligase

Applications:
– Direct cloning
– Ligation-based molecular diagnostic techniques (e.g. Ligase Chain Reaction, LCR; Ligase Detection Reaction, LDR)
– Detection of single-base substitutions

Specifications:

Enzyme concentration: 40 U/μL
Source: Recombinant E. coli strain
Optimal reaction temperature: 55 °C
Heat inactivation: No
Storage conditions: Store at -20 °C
Shipping conditions: Shipped at dry ice

 

Components:
– Taq DNA Ligase (40 U/μL)
– Reaction Buffer (10x)

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