Description: Taq DNA Ligase is a NAD+ dependent thermostable DNA ligase that catalyses the formation of a phosphodiester bond between juxtaposed 5 ́ phosphate and 3 ́ hydroxyl termini of two adjacent nucleotides in the context of a double-stranded DNA molecule. The ligation will occur only if the adjacent nucleotides are perfectly paired and have no gaps between them, thus allowing the use of this enzyme as a tool to detect single-base substitutions. As expected from its origin from a thermophilic host, Taq DNA Ligase is active at elevated temperatures (45–65°C). Due to critical differences in substrate specificity, Taq DNA Ligase is not a substitute for T4 DNA Ligase and it is not suitable for cloning applications or adapter ligation/NGS library prep. Taq DNA Ligase is primarily used in molecular diagnostic applications and for direct cloning applications.
Features:
– Thermostable DNA Ligase
– Active at elevated temperatures (45–65°C)
– Selective ligation of nicks in dsDNA
– Taq DNA Ligase is not a substitute for T4 DNA Ligase
– NAD-dependent DNA ligase
Applications:
– Direct cloning
– Ligation-based molecular diagnostic techniques (e.g. Ligase Chain Reaction, LCR; Ligase Detection Reaction, LDR)
– Detection of single-base substitutions
Specifications:
Enzyme concentration: | 40 U/μL |
Source: | Recombinant E. coli strain |
Optimal reaction temperature: | 55 °C |
Heat inactivation: | No |
Storage conditions: | Store at -20 °C |
Shipping conditions: | Shipped at dry ice |
Components:
– Taq DNA Ligase (40 U/μL)
– Reaction Buffer (10x)