Tetracycline EP

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Tetracycline is a bacteriostatic polyketide antibiotic frequently used in a wide range of in vitro cell culture applications.  TOKU-E offers three forms of tetrcycline: tetracycline HCL (T004), tetracycline, USP (T051), and tetracycline, EP (T016).  Tetracycline, USP and tetracycline, EP are sparingly soluble in aqueous solution at 0.231 mg/mL.  Tetracycline HCl, is slighly soluble in aqueous solution at 10mg/mL.

Mechanism of Action
Tetracycline inhibits protein synthesis by preventing amino-acyl tRNA from binding to the “A” site in the bacterial ribosome.

Spectrum
Tetracycline is a broad spectrum antibiotic targeting a wide range of gram positive and gram negative bacteria responsible for upper respiratory tract infections.

Microbiology Applications
Tetracycline is commonly used in clinical in vitro microbiological antimicrobial susceptibility tests (panels, discs, and MIC strips) against gram positive and gram negative microbial isolates. Medical microbiologists use AST results to recommend antibiotic treatment options for infected patients. Representative MIC values include:
Haemophilus influenzae 6.3 µg/mL – 12.5 µg/mL
Streptococcus pneumoniae 2 µg/mL - 8 µg/mL
For a complete list of tetracycline MIC values, click here.

Plant Biology Applications
Tetracycline has shown to suppress aster yellows disease symptoms on China Aster and Chrysanthemum plants. Multiple applications of tetracycline resulted in symptomless plant growth.

Eukaryotic Cell Culture Applications
Tetracycline is routinely used to select for cells containing resistance plasmids in cell lines such as HeLa at an effective concentration of 1µg/mL. For additional information on your cell culture needs, please visit our cell-culture database.

References
Chopra, Ian, and Marilyn Roberts. "Tetracycline Antibiotics: Mode of Action, Applications, Molecular Biology, and Epidemiology of Bacterial Resistance." Microbiology and Molecular Biology Reviews (2001): 232-60. Http://www.ncbi.nlm.nih.gov. Web. 21 Aug. 2012.

Davis R.E. and Whitcomb, 1970, R.F. Evidence on Possible Mycoplasma Etiology of Aster Yellows Disease. Infection and Immunity, Aug. 1970, p. 201-208