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Transcreener® EPIGEN Methyltransferase Assay (1000 assays, 384-well format)

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The Transcreener® EPIGEN Assay provides universal methyltransferase detection in an High-Throughput Screening (HTS)-proven format. It combines the extensively validated Transcreener® AMP/GMP Assay with coupling enzymes that convert the SAH produced in a Methyltransferase reaction to AMP.


  • Universal: Detects any methyltransferase using any substrate
  • Robust: Z' values greater than 0.7 with low substrate consumption
  • Homogenous Format: Simple mix-and-read format for HTS workflows
  • Sensitive: Reduces enzyme usage and allows use of physiological SAM concentrations
  • >24 hr reagent and signal stability: High quality data in automated workflows at any scale
  • Validated: Optimized with Reaction Biology's purified MT enzymes to eliminate the need for reagent sourcing and assay development


Simple & HTS-Ready
The Transcreener® EPIGEN MT Assay is simple, HTS-ready and has the outstanding data quality and deck and signal stability that Transcreener® Assays are known for. Run your enzyme reaction, add the quench reagent, add detection reagents (coupling enzymes are pre-combined with Transcreener® reagents), and read your plates.


Detection of HMTs with the Transcreener® Epigen Methyltransferase Assay. Enzyme titrations of Set7/9, G9a, and Suv39H1 histone methyltransferases with SAM at the indicated concentrations and a Histone H3-derived peptide as acceptor substrate. .

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