Description: In molecular biology, TBE and TAE buffers are used for agarose and polyacrylamide gel electrophoresis. TAE is advantageous for high resolution of long nucleic acid fragments (longer than 1500 bp) on agarose gels. It has a lower buffering capacity than TBE and in general, nucleic acid fragments move slower in TAE gels (apart from linear dsDNA, which tends to run faster). TAE is also used for native (non-denaturing) RNA analysis and in denaturing gels (instead of MOPS buffer) using prior denaturation of the RNA samples in hot formamide.
Applications:
– Nucleic acid electrophoresis running buffer for agarose and polyacrylamide gels
– Native and denaturing RNA analysis
– Northern blotting
Directions for use: Dilute 20 mL TAE Buffer 50x with 980 mL deionised water to make 1 litre of TAE Buffer 50x
Specifications:
– TRIS 2.0 M 242.0 g/L
– Acetic Acid 1.0 M 60.0 g/L
– Na2EDTA 0.05 M 30.3 g/L
– RNAse Free Solution
– Final concentration is 40 mM Tris base, 20 mM Glacial Acetic Acid and 1 mM Na2EDTA
Storage: Store at room temperature and keep away from light
B49