m-AntiTaq (monoclonal antibody) Clon AHO3
Opis
Monoclonal Antibody to Taq DNA Polymerase: m-Anti-Taq
Enhancer for Polymerase reactions at room temperature / monoclonal antibody to Taq DNA Polymerase
Features
- Anti-Taq (monoclonal antibody to Taq DNA polymerase) inhibits the reaction of Taq DNA Polymerases at room temperature
- Ideal as enhancer for PCR reactions
Application:
Polymerase detergent/enhancer for:
- High specific amplification
- Multiplex amplification
- High sensitivity applications
- Low-copy number PCR
Unit definition:
One unit is defined as the amount required to blocks 50% activity of 1 µg of Polymerase
Note: The ratio units/mg of polymerase varies (up to factor of 10). The binding rate Anti-Taq depends strongly of the polymerization region (active epitopes) of the polymerase. The optimized mixture has to be found in empiric test and for every new lot of polymerase, again.
Storage Buffer: 20mM Tris-HCl (pH 7.0, at 22oC);50 mM KCl;0.1mM EDTA; 50% glycerol
Concentration: 2-10 mg/ml
Units/mg-ratio: 2300 units of the specific polymerase activity are equal to 1 mg of antibodies
QC-Test: > 95% of protein in SDS electrophoresis in 15% PAAG
Storage: at – 20°C
Transportation: at room temperature or with blue ice
References
1. R.T. D'Aquila, L.J. Bechtel, J.A. Videler, Eron JJ, P. Gorczyca, J.C.Kaplan, Maximizing sensitivity and specificity of PCR by pre-amplification heating. Nucleic Acids Res. 19: 3749 (1991)
2. Q. Chou, M. Russell, D.E. Birch, J. Raymond, W. Bloch. Prevention of pre-PCR mis-priming and primer dimerization improves low-copy-number amplifications. Nucleic Acids Res. 20: 1717-23 (1992)
3. D.E. Kellogg , I. Rybalkin, S. Chen, N. Mukhamedova, T. Vlasik, P.D. Siebert, A. Chenchik. TaqStart Antibody: "hot start" PCR facilitated by a neutralizing monoclonal antibody directed against Taq DNA polymerase. Biotechniques. 16: 1134-7 (1994)
Dane techniczne
Opakowanie | 500 µg |