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phi29 DNA Polymerase

nr kat.: EP0091
Opakowanie: 250 U
Cena brutto: do wyceny
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Opis

Supplied with: 0,25 ml of 10X Reaction Buffer

Concentration: 10 u/µl, (0.1 µg/µl) 

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Thermo Scientific phi29 DNA Polymerase is a highly processive polymerase (up to more than 70 kb) featuring strong strand displacement activity, which allows for highly efficient isothermal DNA amplification (see Reference 1). phi29 DNA Polymerase also possesses a 3'→5' exonuclease (proofreading) activity acting preferentially on single-stranded DNA (see Reference 2) or RNA (see Reference 3). Therefore 3'-modified primers are highly recommended (see Reference 4).

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Highlights
  • Highest processivity and strand displacement activity among known DNA polymerases – more than 70 kb long DNA stretches can be synthesized (see Reference 1)
  • Highly accurate DNA synthesis (see Reference 5)
  • Extremely high yields of amplified DNA even from minute amounts of template
  • Amplification products can be directly used in downstream applications (PCR, restriction digestion, SNP genotyping, etc.)
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Applications
......................- amplification of DNA for SNP (see Reference 9) and STR (see Reference 10) detection
......................- cell-free amplification of DNA from single cells (see References 11, 12)
......................- pathogenic organisms or metagenomes (see Reference 13)
......................- amplification of DNA from filter paper blood spot samples (see Reference 14)
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Note
Addition of Pyrophosphatase to the reaction mixture with phi29 DNA Polymerase may enhance DNA synthesis (see Reference 8).
Use of this enzyme in certain applications may be covered by patents and may require a license.

Dane techniczne

Opakowanie 250 U

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