qPCR Master Mix DLP4 (SafeLine: dUTP, UNG, 500 nM ROX)
Opis
For dual labeled probes
Manufactured and quality-controlled in accordance with ISO 9001:2000
Features:
- The Master mix contains dUTP instead of dTTP
- The Master contains UDG (Uracil-Glycosylase)
- The Mix contains ROX (500nM) as passive Reference dye (it provides a baseline in multiplex reactions)
- The qPCR / RTD-PCR Master mix DLP4 is ready-to-use and is optimized for high specificity and
sensitivity because of optimized reaction buffer
- easy to use because ready-to-use Master Mix
Applications:
- Detection and quantification of DNA and cDNA targets
- Profiling gene expression
- Microbial detection
- Viral load determination
Description:
The Master Mix contains all reagents required for qPCR (except template and primer) in a premixed 2x concentrated ready-to-use solution. The high specificity and sensitivity of the mix is achieved by an optimized hot-start polymerase. Its activity is blocked at ambient temperature and switched on automatically at the onset of the initial denaturation. The thermal activation prevents the extension of non-specifically annealed primers and primer-dimer formations at low temperatures during PCR setup.
The mix offer dUTP instead of dTTP and UDG to prevent carry-over contaminations of DNA from previous PCR reactions.
Concentration: The Mastermix is 2x concentrated
List of components qPCR / RTD-PCR Master mix:
Hot-Start Polymerase for qPCR, dATP, dCTP, dGTP, dUTP, ROX, UNG, optimized reaction buffer with KCl and MgCl2, stabilizers and enhancers, PCR-grade water
Storage: at 4°C for 3 months, at -20°C for more than 12 months, Note: protect from light
Dane techniczne
Opakowanie | 100 react. |