qPCR Master Mix EVA1 (SafeLine: EvaGreen, dUTP) for HRM

Manufactured and quality-controlled in accordance with ISO 9001:2000

Features:
 - The Master mix contains dUTP instead of dTTP
 - It contains EvaGreen as fluorescent dye
 - The qPCR / RT-PCR Mastermix E1 is ready-to-use and is optimized for high specificity and sensitivity
   because of optimized reaction buffer
 - easy to use because ready-to-use Master Mix
 - The Master Mix can be used with ROX as reference dye (1x concentrated)

Applications:
 - Detection and quantification of DNA and cDNA targets
 - Profiling gene expression
 - Microbial detection
 - Viral load determination

Compatibility:
BioRad: iCycler, MyiQ, MiQ 2, iQ 5, CFX-96, CFX-384, MJ Opticon, Option2, Chromo4, MiniOpticon
 Qiagen: Roto-Gene Q, Roto-Gene3000, Roto-Gene 6000
 Eppendorf: Mastercycler realplex
 Illumina: Eco RealTime PCR System
 Cepheid: SmartCyler
 Roche: LIghtCycler 480, LightCycler 2.0

Description:
The Master Mix contains all reagents required for qPCR (except template and primer) in a premixed 2x concentrated ready-to-use solution. The high specificity and sensitivity of the mix is achieved by an optimized hot-start polymerase. Its activity is blocked at ambient temperature and switched on automatically at the onset of the initial denaturation. The thermal activation prevents the extension of non-specifically annealed primers and primer-dimer formations at low temperatures during PCR setup.
 The mix contains dUTP instead of dTTP and allows an UNG (Uracil-N-Glycosylase) treatment at the onset of thermal cycling to prevent carry-over contaminations of DNA from previous PCR reactions.
Concentration: The Master mix is 2x concentrated

List of components:
Hot-Start Polymerase for qPCR, dATP, dCTP, dGTP, dUTP, EvaGreen, optimized reaction buffer with KCl and MgCl2, stabilizers and enhancers, PCR-grade water

Storage: at 4°C for 3 months, at -20°C for more than 12 months, Note: protect from Light