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Producenci

siGLO Green Transfection Indicator

nr kat.: D-001630-01-05
Cena brutto: do wyceny
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Producent: Horizon Discovery Group
nr kat.: D-001630-01-05

Opis

siGLO Green Transfection Indicator is a fluorescent oligonucleotide duplex that localizes to the nucleus, thus concentrating its signal to permit unambiguous visual assessment of uptake into mammalian cells. They are ideal for use in optimization experiments to determine ideal siRNA or microRNA reagent transfection conditions and for monitoring relative delivery efficiency during silencing experiments. siGLO Green (6-FAM) Transfection Indicator exhibits nearly 100% signal localization to the nucleus and represents an ideal choice for lipid-mediated transfection optimization.

Transfection Indicators are intended as a qualitative indicator of delivery and should not be used for quantitative determination of siRNA uptake.

Highlights

  •     FAM-labeled Transfection Indicator exhibits nearly 100% signal localization to the nucleus with traditional transfection reagents
  •     Absorbance/Emission Max is 494/520 nm, a FITC filter can be used
  •     Specially modified, fluorescent RNA duplex for simplified transfection assessment - without performing a functional assay
  •     Fluorescent signal localizes to the nucleus as an unmistakable signal of uptake
  •     Use as an indicator of transfection success in any RNAi experiment

 

 

Assessment of nuclear fluorescence permits easy optimization of conditions that result in efficient siRNA delivery into cells. siGLO transfection indicators are specially designed for nuclear localization for a bright, clear signal of transfection success. siGLO Green Transfection Indicator (50 nM) was transfected into HeLa cells with DharmaFECT 1 (0.15 ug/100 uL well). After 24 hours, cells were washed with 1x PBS and stained with Hoechst 3342 nuclear dye (blue).

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