C. elegans Transcription Factor Strain - Yeast
Opis
• Represents ~80% of all predicted C. elegans transcription factors
• Useful for both yeast one-hybrid and two-hybrid assays
• Each construct is available in yeast for mating with bait strains or in an E. coli host for transofrmation into bait strains
We provide certain clone resources developed by leading academic laboratories. Many of these resources address the needs of specialized research communities not served by other commercial entities. In order to provide these as a public resource, we depend on the contributing academic laboratories for quality control.
Therefore, these are distributed in the format provided by the contributing institution "as is" with no additional product validation or guarantee. We are not responsible for any errors or performance issues. Additional information can be found in the product manual as well as in associated published articles (if available). Alternatively, the source academic institution can be contacted directly for troubleshooting.
Clones are provided as a live culture in a 2 mL tube. Each tube contains LB medium supplemented with 8% glycerol and the appropriate antibiotic. Within 2 to 3 business days of receiving your order, we will ship your clone at room temperature via express delivery. Store the stock clone at -80°C.
Orders of 50 clones or greater and stock plates will be provided in microtiter plates. These will ship on dry-ice via overnight delivery and should be stored at -80°C immediately upon receipt.
1.B. Deplancke et al., A Gateway-compatible yeast one-hybrid system. Genome Research. 14, 2093–2101 (2004).
2.Deplancke B et al., A gene-centered C. elegans protein-DNA interaction network.Cell. 125(6), 1193–1205 (2006).
3.V. Vermeirssen et al., A C. elegans transcription factor array and Steiner Triple System-based smart pools: high-performance tools for transcription regulatory network mapping. Nature Methods. 4, 659–664 (2007).
4.A. J. M. Walhout, Unravelling transcription regulatory networks by protein-DNA and protein-protein interaction mapping. Genome Research. 16, 1445–1454 (2006).