Yeast

Saccharomyces cerevisiae is a powerful model for understanding genetic regulation in eukaryotic cells. Dharmacon yeast resources include multiple collections for interrogating the yeast genome, including Tagged ORFs, Kockout Strains, Protein-Protein Interaction Collections and Mutant Strains and Screening Collections.

Tagged ORFs

• Yeast ORF Collection
  The Yeast ORF collection consists of Gateway-adapted yeast ORFs cloned into expression plasmids. ORFs can be expressed as fusion proteins or moved to other vectors.
• Yeast YFP Fusion Kinase Collection
  The Yeast Kinase YFP Fusion collection contains a number of kinase genes that have been cloned with a C-terminal YFP tag and promoter region into a Gatewa-entry vector.
• Yeast TAP Tagged ORFs
  Yeast-TAP Tagged ORF library allows the purification and the selection of the yeast proteome and associated components using two affinity selection steps in tandem.
• Molecular Barcoded Yeast (MoBY) ORF Collection
  The Molecular Barcoded Yeast (MoBY) ORF library was designed to identify genes in Saccharomyces cerevisiae that confer drug resistance when mutated, providing a tool for identifying drug-resistant mutations in yeast.
• Yeast GST-tagged ORFs
  The Yeast GST-tagged ORFs are a a collection of more than 5,000 yeast strains that each overexpresses a different yeast open reading frame (ORF) when induced with galactose.
• Yeast HA Tag Collection
  The Yeast HA-Tagged Collection contains mutagenized yeast strains with triple hemagglutinin (HA) epitope tags, useful for immunolocalization, immunoprecipitation, and analysis of binding sites.
• Yeast Barcoder Collection
  The Yeast Barcoder Collection is a set of approximately 1,400 donor strains with unique DNA sequence tags (barcodes) that can be systematically transferred to any S. cerevisiae collection.

Knockout Strains

• Yeast Knockout Collection
  The Yeast Knockout (YKO) Collection contains over 6,000 gene-disruption mutants as a unique tool for the functional analysis of the yeast genome.
• Yeast Tet-promoters Hughes Collection (yTHC)
  The Yeast Tet-Promoters Hughes Collection (yTHC) with 800 essential yeast genes under control of a tetracycline-regulated promoter permits experimental regulation of essential genes.
• YKO Parental Strains
  Parental yeast strains for use with the Yeast Knockout (YKO) and other yeast collections.

Protein-Protein Interaction Collections

• Yeast Protein Interactome Collection
  The Yeast Protein Interactome Collection allows for genome-wide in vivo screening of protein-protein interactions (PPI) in Saccharomyces cerevisiae.
• Yeast Cross and Capture System
  The Yeast Cross and Capture system is designed to examine protein-protein interactions and protein modifications using 6xHIS and VSV tagged haploid yeast strains.

Mutant Strains and Screening Collections

• Yeast Insertional Mutant Collection
  The Yeast Insertional Mutant collection contains a set of over 3,600 gene disruption mutants for phenotypic analysis.
• Yeast Synthetic Histone Collection
  Systematic mutations have been engineered into the Yeast Synthetic Histone H3 and H4 mutants in order to elucidate nucleosome functionality.
• Yeast Genomic Tiling Collection
  Designed to facilitate comprehensive overexpression screens, the Yeast Genomic Tiling Collection contains the yeast (Saccharomyces cerevisiae) genome in a 2 micron-based LEU2 vector.
• Yeast DAmP Collection
  Reduce mRNA levels of essential yeast genes by four- to ten-fold with the Decreased Abundance by mRNA Perturbation (DAmP) Yeast Library.

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