

CpG Methyltransferase (M.SssI)
Opis
Features
- High efficiency – complete in vitro methylation of all CpGs in non-methylated and hemi-methylated DNA.
- Fast – reaction is completed in 15 min at 37°C.
- Stable – at -20°C for 2 years.
- Epigenetics studies.
- In vitro methylation of DNA for methylation analysis.
- Inhibition of endonucleases with overlapping CpG sequence recognition.
- [3H]-labeling of DNA.
Thermo Scientific M.SssI is specifically formulated for fast restriction times without compromising the reaction efficiency. The enzyme completes modification of all CpGs in 15 min at 37°C. In addition, the enzyme has been specifically validated for use with genomic DNA – the primary substrate in epigenetic studies.
10 mM potassium phosphate (pH 7.0 at 25°C), 400 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.2 mg/ml BSA and 50% glycerol.
No detectable change in DNA fragment band pattern observed following agarose gel electrophoresis of SmaI linearized pUC19 DNA incubated for 16 hours with 1 µl of M.SssI.
Labeled Oligonucleotide (LO) Assay
No detectable degradation of single-stranded or double-stranded labeled oligonucleotides observed after incubation with 1 µl of M.SssI for 4 hours at 37°C, analysis by polyacrylamide gel electrophoresis and phosphoimaging
Dane techniczne
Opakowanie | 25 µl |