Produkty
Producenci
p3T, PCR Product Cloning Vector, lyophilized DNA
nr kat.:
P123T
Opakowanie:
5 µg
Producent:
nr kat.:
P123T
Opis
Features
Description
The p3T vector provides a flexible system for the direct cloning of PCR products. It allows cloning of PCR products via multiple dT overhangs. Utilizing the restriction site PflMI, three dT overhangs are produced. After polyadenylating the PCR fragment with Terminal Deoxynucleotidyl Transferase, the PCR fragment can be cloned with high efficiency via a multiple dA extension.
Using p3T, less amplified DNA is required. A SmaI site is present to reduce vector background; MscI sites flank the insert for optimal excision. Blue/white selection by alpha -complementation is possible.
- Cloning PCR products via multiple dA extensions
- Results in higher cloning efficiencies than with single dA/dT overhangs
Description
The p3T vector provides a flexible system for the direct cloning of PCR products. It allows cloning of PCR products via multiple dT overhangs. Utilizing the restriction site PflMI, three dT overhangs are produced. After polyadenylating the PCR fragment with Terminal Deoxynucleotidyl Transferase, the PCR fragment can be cloned with high efficiency via a multiple dA extension.
Using p3T, less amplified DNA is required. A SmaI site is present to reduce vector background; MscI sites flank the insert for optimal excision. Blue/white selection by alpha -complementation is possible.
Dane techniczne
| Opakowanie | 5 µg |