UV
Tests
NZYTech test kits are based on enzymatic reactions and performed
using spectrophotometric methods.
The principle of the enzymatic tests is based on the
NAD(P)+/NAD(P)H system and uses highly pure enzymes engineered to
display a premium performance. The enzymes used in these
analytical kits produce or consume NAD(P)H, which strongly
absorbs the UV radiation at 340 nm (extinction coefficient of
6300 M-1cm-1).
Colorimetric Tests
The principle of the enzymatic tests based on a chromogenic reaction is the formation of a coloured compound, which absorbs at the visible region of the spectrum. The coloured compound results from the interaction between the product of a first enzymatic reaction and a chromogenic compound. In this case, the concentration of the analyte must be determined by using a standard curve.
- Endpoint analysis
- Easy to use, simple protocols
- Rapid analysis
- Accuracy and precision
- Safe to the operator
- Standards included
Required Materials
- Spectrophotometer
- Micropipettes set with disposable plastic tips to accurately dispense volumes from 20 μL to 1000 μL
- Cuvettes
- Basic filtering or other simple sample treatment device