Hygromycin B EvoPure Solution (100 mg/ml in PBS Buffer)

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Hygromycin B, EvoPure® solution (100 mg/mL) is a solution containing high purity (>99.0%) hygromycin B. Hygromycin B is a unique aminoglycoside antibiotic derived from Streptomyces hygroscopicus and is routinely used as a selective agent in cell culture or microbiology applications to isolate hygromycin B resistant cells after transfection or transformation, respectively.

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Molecular Formula: C₂₀H₃₇N₃O₁₃

Molecular Weight: 527.52 g/mol

Mechanism of Action
Hygromycin B inhibits protein synthesis by strengthening the interaction of tRNA binding in the ribosomal A-site. Hygromycin B also prevents mRNA and tRNA translocation by an unknown mechanism. These are unique mechanisms for an aminoglycoside antibiotic and they differ from the mode of action neomycin, gentamicin, and G418.


Spectrum
Hygromycin B is effective against eukaryotic and prokaryotic cells.

Eukaryotic Cell Culture Applications

Hygromycin B is routinely used as a selective agent in mammalian cell culture to isolate hygromycin B resistant cells after transfection. Selectable markers for hygromycin B resistant cells include the hyg or hph resistance genes which express a phosphotransferase that inactivates hygromycin B by phosphorylation. Effective working concentrations range from 100 – 1000 µg/mL.

For additional information regarding relevant cell lines, resistance plasmids, and culture media, please visit our cell culture database.

Microbiology Applications

Hygromycin B can be used as a selection agent to isolate hygromycin b resistant bacteria and fungi.

References

Dai S., Zheng P., Marmey P., Zhang S., Tian W., Chen S., Beachy R.N. and Fauquet C. Comparative analysis of transgenic rice plants obtained by Agrobacterium-mediated transformation and particle bombardment. Molecular Breeding 7: 25–33, 2001. © 2001 Kluwer Academic Publishers.

Schindler, D. "Studies on the Mode of Action of Hygromycin B, an Inhibitor of Translocation in Eukaryotes." Nucleic Acids and Protein Synthesis 521.2 (1978): 459-69. www.ncbi.gov. Web. 6 Sept. 2012.