- standard cloning into a site upstream from a T7-RNA-polymerase-promoter
- linearization of recombinant clones
- blue/white selection
- generation of single-strand DNA (using the f1 origin)
The new cloning vector pMCS5 contains the ultimate multiple cloning site (MCS) with 59 unique restriction sites. Included are the recognition sites for many commonly used restriction enzymes, providing a suitable cloning site for almost any application.
The scientist can chose between 46 unique hexamer sites, two heptamer sites and the recognition sites for all ten known octanucleotide-specific endonucleases. In addition, the well-defined 18-mer sequence of the extremely rarely cutting enzyme I-Sce I is located at one terminus of the MCS, enabling the linearization of recombinant clones. I-Sce I does not recognize a palindrome and can thus be used for labeling and end-cloning. Further it can be utilized for strand protection in unidirectional deletion experiments. Included in the MCS are also restriction sites which occur very infrequently in human DNA such as Mlu I, Nru I and Spl I.