Nalidixic Acid Sodium CulturePure®

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Nalidixic acid is a naphthyridone antibiotic similar in structure and mechanism to quinolones.

TOKU-E offers two forms of nalidixic acid: nalidixic acid (N011) and nalidixic acid sodium salt, CulturePure^® (N001). Nalidixic acid is sparingly soluble in aqueous solution at 0.1 mg/mL. Nalidixic acid sodium salt is freely soluble in aqueous solution at 50 mg/mL.

Cas Number: 3374-05-8

Molecular Formula: C₁₂H₁₁N₂NaO₃

Molecular Weight: 254.22

Mechanism of Action: Nalidixic acid indirectly inhibits proteins synthesis by binding DNA and interfering with enzymes involved in DNA replication and transcription.

Spectrum: Nalidixic acid is a broad spectrum antibiotic commonly used against bacteria responsible for urinary tract infections.

Microbiology Applications: Nalidixic acid is commonly used in clinical in vitromicrobiological antimicrobial susceptibility tests (panels, discs, and MIC strips) against gram positive and gram negative microbial isolates. Medical microbiologists use AST results to recommend antibiotic treatment options for infected patients. Representative MIC values include:

• Escherichia coli 2 µg/mL - 8 µg/mL
• Proteus mirabilis 2.1 µg/mL - 8 µg/mL
• For a complete list of nalidixic acid MIC values, click here.

Kiianitsa et al. used TOKU-E nalidixic acid at 100 µg/mL to study DNA gyrase inhibition in E. coli K-12. "Ultrasensitive isolation, identification and quantification of DNA–protein adducts by ELISA-based RADAR assay" 

Media Supplements

Nalidixic acid can be used as a selective agent in several types of isolation media:

Columbia Blood Agar - Staphylococcus/Streptococcus selective supplement

Wilkins-Chalgren Anaerobe Agar - Isolation of non-sporing anaerobes

Wilkins-Chalgren Anaerobe Agar - Isolation of Gram-negative anaerobes

Columbia Blood Agar - Gardnerella vaginalis Selective Supplement

Listeria Enrichemnt Broth - Listeria Selective Enrichment Supplement

Listeria Enrichemnt Broth - Listeria Selective Enrichment Supplements UVM I and UVM II

Listeria Enrichment Broth - Modified Listeria Selective Enrichemnt Supplement

Fraser Broth - Fraser Supplement

Fraser Broth - Half Fraser Supplement

Brucella Medium Base - Modified Brucella Selective Supplement

Buffered Listeria Enrichment Broth - Listeria Selective Enrichment Supplement

Source: Synthetic

Water Content (Karl Fischer): ≤7.0%

References:

Levitt, Martin, and et al. "Studies on the Mechanism of Action of Nalidixic Acid." Studies on the Mechanism of Action of Nalidixic Acid 4.4 (1973): 479-86. www.ncbi.gov. Web. 10 Sept. 2012.