Produkty
Producenci
Neomycin Sulfate EP
Opis
Produkt przeznaczony wyłącznie do badań. Nie nadaje się do spożycia. Więcej informacji na stronie producenta www.toku-e.com
Neomycin Sulfate, EP is a broad-spectrum aminoglycoside antibiotic composed of related compounds including Neomycin A (neamine), Neomycin B (framycetin), Neomycin C, and a few minor compounds. Neomycin B is the most active, followed by C and A. The quantities of these components vary from lot-to-lot.
Neomycin Sulfate, EP conforms to European Pharmacopoeia specifications.
For additional Neomycin products, click here.
Custom Manufacturing and Testing: We are able to prepare custom Neomycin Sulfate suitable for your unique specifications for use in cell culture, or as ancillary material in upstream biopharma manufacturing. Additionally, we offer additional testing including endotoxin content, arsenic content, cell line testing, spectral analysis, and more. For more information, please contact us.
Cas Number: 1405-10-3
Molecular Formula: C23H46N6O13 ·3 H2SO4
Molecular Weight: 908.88
Mechanism of Action: Aminoglycosides target the 30S ribosomal subunit resulting in an inability to read mRNA ultimately producing a faulty or nonexistent protein.
Storage Conditions: 2-8°C
Application
Spectrum: Neomycin is a broad spectrum antibiotic, however, it is mostly used against Gram-negative bacteria.
Microbiology Applications:
Neomycin can be used for gene selection, via exploiting the resistance gene (NPT II) (Aragão, 2009).
Neomycin is commonly used in clinical in vitro microbiological antimicrobial susceptibility tests (panels, discs, and MIC strips) against Gram-positive and Gram-negative microbial isolates. Medical microbiologists use AST results to recommend antibiotic treatment options. Representative effective ranges include:
- Pseudomonas aeruginosa 0.5 µg/mL – 64 µg/mL
Haemophilus influenzae 1.6 µg/mL – 6.3 µg/mL
For a representative list of Neomycin MIC values, click here.
Neomycin Sulfate can be used for food testing in TSN agar to select for Clostridium perfringins and inhibit growth of Enterobacteria and Clostridium bifermentans.Plant Biology Applications: Neomycin is commonly used in negative selections for plants which have been successfully been transformed with a plasmid conferring resistance via Agrobacterium-mediated transformation
Eukaryotic Cell Culture Applications: Neomycin is routinely used to select for cells containing resistance plasmids such as pcDNA3 in common cell lines including AtT-20. For complete additional information on your cell culture needs, please visit our cell-culture database.
Specification
Form: Powder
Appearance: White or yellowish powder
Source: Streptomyces fradiae.
pH: 5.0-7.5
Assay: (Dried Basis): ≥680IU/mg
Loss on Drying: ≤8.0%
Optical Rotation: +53.5° to +59.4°
Solubility: Ethanol: 0.10 mg/mL
Methanol: 0.23 mg/mL
Water: 50 mg/mL
Elemental Analysis: Sulphate: 27.0-31.0%
Impurity Profile: Impurity A| 2-deoxy-4-O-(2,6-diamino-2,6-dideoxy-d-glucopyranosyl)-d-streptamine (neamine or neomycin A-LP)|||| Impurity B| 3-N-acetyl-2-deoxy-4-O-(2,6-diamino-2,6-dideoxy-d-glucopyranosyl)-d-streptamine (3-acetylneamine)|||| Impurity C| 4-O-(2-amino-2-deoxy-d-glucopyranosyl)-2-deoxy-d-streptamine (paromamine or neomycin D)|||| Impurity D| 2-deoxy-4-O-(2,6-diamino-2,6-dideoxy-d-glucopyranosyl)-5-O-[3-O-(2,6-diamino-2,6-dideoxy-d-glucopyranosyl)-d-ribofuranosyl]-d-streptamine (neomycin C)|||| Impurity E| 4-O-(2-amino-2-deoxy-d-glucopyranosyl)-2-deoxy-5-O-[3-O-(2,6-diamino-2,6-dideoxy-l-idopyranosyl)-d-ribofuranosyl]-d-streptamine (paromomycin I or neomycin E)|||| Impurity F| 4-O-(2-amino-2-deoxy-d-glucopyranosyl)-2-deoxy-5-O-[3-O-(2,6-diamino-2,6-dideoxy-d-glucopyranosyl)-d-ribofuranosyl]-d-streptamine (paromomycin II or neomycin F)|||| Impurity G| 3-N-acetyl-2-deoxy-4-O-(2,6-diamino-2,6-dideoxy-d-glucopyranosyl)-5-O-[3-O-(2,6-diamino-2,6-dideoxy-l-idopyranosyl)-d-ribofuranosyl]-d-streptamine (neomycin B-LP)||||
Dane techniczne
| Opakowanie | 100 g |