Produkty
Producenci
Nitrocefin
Opis
Nitrocefin is a cephalosporin with chromogenic properties and is routinely used to detect beta-lactamase enzymes produced by beta-lactam resistant bacteria. Nitrocefin is soluble in DMSO and is commonly used at a 1.0 mg/mL concentration.
Mechanism
Essentially all beta-lactamase enzymes hydrolyze the amide bond between the carbonyl carbon and the nitrogen in the beta-lactam ring of nitrocefin. Macroscopic detection of this process is made possible because a ultraviolet absorption shift from intact versus hydrolyzed nitrocefin occurs within the visible light spectrum (~380 nm to ~500 nm, or yellow to red).
Microbiology Applications
Nitrocefin is used to detect beta-lactamase activity from suspected beta-lactam resistant bacteria (see protocol below).
Form: Powder
Purity Level: (HPLC): ≥95.0%
Produkt przeznaczony wyłącznie do badań. Nie nadaje się doa spożycia.
Dane techniczne
| Opakowanie | 5 x 10 mg |