Tetracycline Hydrochloride EP/USP

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Tetracycline is a bacteriostatic polyketide antibiotic frequently used in a wide range of in vitro cell culture applications.

TOKU-E offers three forms of tetrcycline: tetracycline HCL (T004), tetracycline, USP (T051), and tetracycline, EP (T016). Tetracycline, USP and tetracycline, EP are sparingly soluble in aqueous solution at 0.231 mg/mL.  Tetracycline HCl, is slighly soluble in aqueous solution at 10mg/mL.

CAS Number: 64-75-5

Molecular Formula: C₂₂H₂₄N₂O₈ •HCl

Molecular Weight: 480.90

Mechanism of Action
Tetracycline HCl inhibits protein synthesis by preventing amino-acyl tRNA from binding to the “A” site in the bacterial ribosome.

Spectrum
Tetracycline HCl is a broad spectrum antibiotic targeting a wide range of gram positive and gram negative bacteria responsible for upper respiratory tract infections.

Eukaryotic Cell Culture Applications
Tetracycline is routinely used to select for cells containing resistance plasmids in cell lines such as HeLa at an effective concentration of 1µg/mL. For additional information on your cell culture needs, please visit our cell-culture database.
   
Microbiology Applications
Tetracycline HCl is routinely used as a selective agent to select for bacterial cells that have been transformed with a plasmid that contains the tetracycline resistance gene, tet. Tetracycline is typically used at 10 µg/mL.

Plant Biology Applications
Tetracycline has shown to suppress aster yellows disease symptoms on China Aster and Chrysanthemum plants. Multiple applications of tetracycline resulted in symptomless axillary plant growth (Davis R.E. and Whitcomb, 1970).

Impurity Profile
4-Epitetracycline: Not more than 3.0%
Anhydrotetracycline: Not more than 0.5%
4-Epianhydrotetracycline: Not more than 0.5%
2-Acetyl-2-decarbamoyl-tetracycline: Not more than 1.5%
Heavy Metals: ≤50ppm
Sulphate Ash: ≤0.5%
Bacterial Endotoxin: ≤0.5 IU/mg

References
Chopra, Ian, and Marilyn Roberts. "Tetracycline Antibiotics: Mode of Action, Applications, Molecular Biology, and Epidemiology of Bacterial Resistance." Microbiology and Molecular Biology Reviews (2001): 232-60. Http://www.ncbi.nlm.nih.gov. Web. 21 Aug. 2012.

Davis R.E. and Whitcomb, 1970, R.F. Evidence on Possible Mycoplasma Etiology of Aster Yellows Disease. Infection and Immunity, Aug. 1970, p. 201-208